Regeneration of Pea (Pisum sativum L.) by a cyclic organogenic system

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)


In a five-step procedure, plants were regenerated from meristematic tissue initiated from nodal tissue in four pea cultivars ('Espace', 'Classic', 'Solara', and 'Puget'). In step 1, stem tissue with one node (1-cm size) was subcultured on medium containing thidiazuron. As a result multiple shoots were produced, appearing normal or swollen at their bases. The multiple shoots were subcultured in the same medium, resulting in the formation of a green hyperhydric tissue in the swollen bases of the multiple shoots, which is fully covered with small buds [bud-containing tissue (BCT)]. In step 2, BCT fragments were isolated and subcultured in the same medium and, as a result, they were able to reproduce themselves in a cyclic fashion. In step 3, subculture of BCT on medium supplemented with a combination of gibberelic acid, 6-benzyladenine and a-naphthalene acetic acid (NAA), resulted in the formation of shoots, which were rooted in step 4 on medium supplemented with 0.5 mg/l NAA, indole-3-acetic acid (IAA) or indole-3-butyric acid. In step 5, in vitro plants were transferred to the greenhouse for acclimatisation and further development. The four varieties tested were all able to produce meristematic tissue, suggesting that its production is genotype independent.
Original languageEnglish
Pages (from-to)453-461
JournalPlant Cell Reports
Issue number7
Publication statusPublished - 2004


  • somatic embryogenesis
  • transformation
  • thidiazuron
  • cultures
  • embryos
  • growth

Fingerprint Dive into the research topics of 'Regeneration of Pea (Pisum sativum L.) by a cyclic organogenic system'. Together they form a unique fingerprint.

Cite this