Regeneration and transformation of cassava (Manihot esculenta Crantz.)

E. Sofiari

Research output: Thesisinternal PhD, WU

Abstract

This thesis describes different regeneration systems of cassava. In the first system the embryos were highly organized. The use of the auxins NAA and 2,4-D to induce this organized system of somatic embryogenesis were compared in several genotypes. Bombardment of organized tissues did not result in transformed plants and culture of protoplasts isolated from organized cultures did not result in plant regeneration. In the second system, so called friable embryogenic callus, the embryos are less organized. Protoplasts isolated from friable embryogenic callus regenerated into plants. Bombardment of this friable embryogenic callus with DNA of constructs containing the luciferase gene resulted in transformed tissue. Transgenic tissue was selected using luciferase activity. Transformed mature embryos were multiplied by the organized system of embryogenesis before they were allowed to develop into plants. The transformed nature of the plant was confirmed by PCR and Southern Blot Analysis.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Jacobsen, Evert, Promotor
  • Visser, Richard, Promotor
Award date28 May 1996
Place of PublicationS.l.
Publisher
Print ISBNs9789054855361
Publication statusPublished - 1996

Keywords

  • cassava
  • manihot esculenta
  • genetic transformation
  • tissue culture
  • somatic embryogenesis
  • plant breeding

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