Regeneration and genetic transformation in the Vitis genus = [Regeneratie en genetische transformatie in het genus Vitis =

L. Martinelli

Research output: Thesisexternal PhD, WU

Abstract

<br/>This work is a contribution to the development of regeneration systems and genetic transformation in the <em>Vitis</em> genus and opens interesting perspectives to the application of molecular techniques for study interesting traits, as well as for genetic improvement of grape.<p>Somatic embryogenesis from leaf and petioles has been obtained in <em>V.</em><em>rupestris S.</em> and the most crucial steps of this morphogenic event have been characterized through a two-dimensional electrophoretic analysis of the total proteins and a study of isozyme patterns of five enzymes.<p>In this genotype, the co-culture of isolated somatic embryos during' secondary embryogenesis induction with <em>Agrobacterium tumefaciens</em> (LBA4404), which contains the plasmid pBI121 carrying the neomycin-phosphotranspherase and the β-glucuronidase genes, proved to be a valuable strategy for the production of stably transgenic plants.<p>The regeneration potential in the <em>Vitis</em> genus during direct organogenesis from young leaves was conducted on 18 different <em>Vitis</em> genotypes, including cultivars of <em>Vitis vinifera L.,</em> wild species, rootstocks and a hybrid variety was investigated, opens the possibility to employ <em>in vitro</em> strategies for molecular studies and genetic improvement to valuable grape genotypes too.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Koornneef, Maarten, Promotor
Award date22 Dec 1997
Place of PublicationS.l.
Publisher
Print ISBNs9789054857679
Publication statusPublished - 1997

Keywords

  • recombination
  • crossing over
  • vitis
  • grapes

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