Reduction of cell size induced by enod40 in Arabidopsis thaliana

F. Guzzo, P. Portaluppi, R. Grisi, S. Barone, S. Zampieri, H. Franssen, M. Levi

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

An extensive analysis of organ and cell size was performed in three different Arabidopsis lines transformed with the early nodulin gene enod40 under control of the CaMV35S promoter. All three transgenic lines presented a significant decrease in the mean size of both epidermal internode and leaf mesophyll cells. Flow cytometric and image analysis of enod40-transfected protoplasts prepared from wild-type Arabidopsis cell suspensions showed that transient expression of the gene resulted in reduced forward light scattering (a factor correlated with particle size) and cell size. The direct administration of ENOD40 peptide to fresh protoplasts also resulted in reduced forward scattering with respect to the control and to the administration of unrelated peptides. As far as is known this is the first report documenting a biological effect of enod40 at the cellular level in non-legume plants
Original languageEnglish
Pages (from-to)507-513
JournalJournal of Experimental Botany
Volume56
Issue number412
DOIs
Publication statusPublished - 2005

Fingerprint

Protoplasts
Cell Size
Arabidopsis
Arabidopsis thaliana
Mesophyll Cells
Peptides
protoplasts
Organ Size
Particle Size
peptides
nodulins
Suspensions
light scattering
cells
Gene Expression
Light
internodes
mesophyll
cell suspension culture
particle size

Keywords

  • soybean nodule development
  • plant development
  • sucrose synthase
  • gene-expression
  • flow-cytometry
  • growth
  • division
  • cultures
  • rna
  • organogenesis

Cite this

Guzzo, F., Portaluppi, P., Grisi, R., Barone, S., Zampieri, S., Franssen, H., & Levi, M. (2005). Reduction of cell size induced by enod40 in Arabidopsis thaliana. Journal of Experimental Botany, 56(412), 507-513. https://doi.org/10.1093/jxb/eri028
Guzzo, F. ; Portaluppi, P. ; Grisi, R. ; Barone, S. ; Zampieri, S. ; Franssen, H. ; Levi, M. / Reduction of cell size induced by enod40 in Arabidopsis thaliana. In: Journal of Experimental Botany. 2005 ; Vol. 56, No. 412. pp. 507-513.
@article{4fa01ac5cb3544d3bfcce36ddbc2f2ab,
title = "Reduction of cell size induced by enod40 in Arabidopsis thaliana",
abstract = "An extensive analysis of organ and cell size was performed in three different Arabidopsis lines transformed with the early nodulin gene enod40 under control of the CaMV35S promoter. All three transgenic lines presented a significant decrease in the mean size of both epidermal internode and leaf mesophyll cells. Flow cytometric and image analysis of enod40-transfected protoplasts prepared from wild-type Arabidopsis cell suspensions showed that transient expression of the gene resulted in reduced forward light scattering (a factor correlated with particle size) and cell size. The direct administration of ENOD40 peptide to fresh protoplasts also resulted in reduced forward scattering with respect to the control and to the administration of unrelated peptides. As far as is known this is the first report documenting a biological effect of enod40 at the cellular level in non-legume plants",
keywords = "soybean nodule development, plant development, sucrose synthase, gene-expression, flow-cytometry, growth, division, cultures, rna, organogenesis",
author = "F. Guzzo and P. Portaluppi and R. Grisi and S. Barone and S. Zampieri and H. Franssen and M. Levi",
year = "2005",
doi = "10.1093/jxb/eri028",
language = "English",
volume = "56",
pages = "507--513",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "412",

}

Guzzo, F, Portaluppi, P, Grisi, R, Barone, S, Zampieri, S, Franssen, H & Levi, M 2005, 'Reduction of cell size induced by enod40 in Arabidopsis thaliana' Journal of Experimental Botany, vol. 56, no. 412, pp. 507-513. https://doi.org/10.1093/jxb/eri028

Reduction of cell size induced by enod40 in Arabidopsis thaliana. / Guzzo, F.; Portaluppi, P.; Grisi, R.; Barone, S.; Zampieri, S.; Franssen, H.; Levi, M.

In: Journal of Experimental Botany, Vol. 56, No. 412, 2005, p. 507-513.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Reduction of cell size induced by enod40 in Arabidopsis thaliana

AU - Guzzo, F.

AU - Portaluppi, P.

AU - Grisi, R.

AU - Barone, S.

AU - Zampieri, S.

AU - Franssen, H.

AU - Levi, M.

PY - 2005

Y1 - 2005

N2 - An extensive analysis of organ and cell size was performed in three different Arabidopsis lines transformed with the early nodulin gene enod40 under control of the CaMV35S promoter. All three transgenic lines presented a significant decrease in the mean size of both epidermal internode and leaf mesophyll cells. Flow cytometric and image analysis of enod40-transfected protoplasts prepared from wild-type Arabidopsis cell suspensions showed that transient expression of the gene resulted in reduced forward light scattering (a factor correlated with particle size) and cell size. The direct administration of ENOD40 peptide to fresh protoplasts also resulted in reduced forward scattering with respect to the control and to the administration of unrelated peptides. As far as is known this is the first report documenting a biological effect of enod40 at the cellular level in non-legume plants

AB - An extensive analysis of organ and cell size was performed in three different Arabidopsis lines transformed with the early nodulin gene enod40 under control of the CaMV35S promoter. All three transgenic lines presented a significant decrease in the mean size of both epidermal internode and leaf mesophyll cells. Flow cytometric and image analysis of enod40-transfected protoplasts prepared from wild-type Arabidopsis cell suspensions showed that transient expression of the gene resulted in reduced forward light scattering (a factor correlated with particle size) and cell size. The direct administration of ENOD40 peptide to fresh protoplasts also resulted in reduced forward scattering with respect to the control and to the administration of unrelated peptides. As far as is known this is the first report documenting a biological effect of enod40 at the cellular level in non-legume plants

KW - soybean nodule development

KW - plant development

KW - sucrose synthase

KW - gene-expression

KW - flow-cytometry

KW - growth

KW - division

KW - cultures

KW - rna

KW - organogenesis

U2 - 10.1093/jxb/eri028

DO - 10.1093/jxb/eri028

M3 - Article

VL - 56

SP - 507

EP - 513

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 412

ER -