Reduced immune reaction prevents immunopathology after challenge with avian influenza virus: A transcriptomics analysis of adjuvanted vaccines

Sylvia S. Reemers, Christine Jansen, Marian J. Groot Koerkamp, Daphne van Haarlem, Peter van de Haar, Winfried G.J. Degen, Willem van Eden, Lonneke Vervelde*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

To gain more insight in underlying mechanisms correlating to protection against avian influenza virus (AIV) infection, we investigated correlates of protection after AIV H9N2 infection and studied the contribution of different adjuvants to a protective response at host transcriptional level. One-day-old chickens were immunised with inactivated H9N2 supplemented with w/o, Al(OH)3, CpG or without adjuvant. Two weeks later, birds were homologously challenged and at 1-4 days post challenge (d.p.c.) trachea and lung were collected. Birds immunised with H9N2+w/o or H9N2+Al(OH)3 were protected against challenge infection and had lower viral RNA expression, less immune related genes induced after challenge, a lower amplitude of change of gene expression and smaller cellular influxes compared to the higher and prolonged gene expression in unprotected birds. We show that a limited number of differentially expressed genes correlates with reduced immune activation and subsequently reduced immunopathology after challenge with AIV.
Original languageEnglish
Pages (from-to)6351-6360
Number of pages10
JournalVaccine
Volume28
Issue number38
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

Keywords

  • Avian influenza virus
  • Microarray
  • Vaccination

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