Redirection of auxin flow in Arabidopsis thaliana roots after infection by root-knot nematodes

Tina Kyndt, Aska Goverse, Annelies Haegeman, Sonja Warmerdam, Cecilia Wanjau, Mona Jahani, Gilbert Engler, Janice De Almeida Engler, Godelieve Gheysen

Research output: Contribution to journalArticleAcademicpeer-review

29 Citations (Scopus)

Abstract

Plant-parasitic root-knot nematodes induce the formation of giant cells within the plant root, and it has been recognized that auxin accumulates in these feeding sites. Here, we studied the role of the auxin transport system governed by AUX1/LAX3 influx proteins and different PIN efflux proteins during feeding site development in Arabidopsis thaliana roots. Data generated via promoter–reporter line and protein localization analyses evoke a model in which auxin is being imported at the basipetal side of the feeding site by the concerted action of the influx proteins AUX1 and LAX3, and the efflux protein PIN3. Mutants in auxin influx proteins AUX1 and LAX3 bear significantly fewer and smaller galls, revealing that auxin import into the feeding sites is needed for their development and expansion. The feeding site development in auxin export (PIN) mutants was only slightly hampered. Expression of some PINs appears to be suppressed in galls, probably to prevent auxin drainage. Nevertheless, a functional PIN4 gene seems to be a prerequisite for proper nematode development and gall expansion, most likely by removing excessive auxin to stabilize the hormone level in the feeding site. Our data also indicate a role of local auxin peaks in nematode attraction towards the root
Original languageEnglish
Pages (from-to)4559-4570
JournalJournal of Experimental Botany
Volume67
Issue number15
DOIs
Publication statusPublished - 2016

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