Recovery of a bioactive tripeptide from a crude hydrolysate using activated carbon

M. Rodriguez Illera*, A. Ramires Ferreira Da Silva, R.M. Boom, A.E.M. Janssen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)


Separation and purification processes of neutraceuticals, such as bioactive peptides, are usually done in a multistep process that sometimes requires a final chromatographic step using expensive resins. Activated carbon is a promising economic alternative for the resins. We report here on the application of a hydrophobic interaction on a chromatographic column packed with particles of activated carbon to isolate a lactotripeptide from a crude hydrolysate. Consecutive adsorptive–desorptive cycles were used until exhaustion of the column. Liquid chromatography–mass spectrometry results showed an enrichment of the lactotripeptide isoleucine–proline–proline with a yield of up to 80% in the third cycle and a twofold increase in purity to up to 35%. Some guidelines are given for the competitive exhaustion of the adsorbent for process optimization in order to obtain higher purity and yield.
Original languageEnglish
Pages (from-to)255-262
JournalFood and Bioproducts Processing
Publication statusPublished - 2015


  • hydrophobic interaction chromatography
  • high fischer ratio
  • enzyme-inhibitory properties
  • pore-size distribution
  • competitive adsorption
  • protein hydrolysate
  • binding-capacity
  • peptide mixture
  • antioxidant
  • water


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