Recombinant Newcastle disease viruses with targets for PCR diagnostics for rinderpest and peste des petits ruminants

P.A. van Rijn, J. Boonstra, H.G.P. van Gennip

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Since February 1st 2011, rinderpest (RP) has been officially declared eradicated worldwide. National authorities have been requested to destroy all their RP related materials. Nonetheless, their national reference laboratories performing real time reverse transcription polymerase chain reaction assays (PCR diagnostics) need RP positive control samples, since some countries still prefer to maintain diagnostic capability for RP for several reasons. In the future, a similar situation will arise for peste des petits ruminants (PPR) as the ambition has been expressed to eradicate PPR. Anticipating on this, we intended to perform qualified PCR diagnostics without use of infectious RPV or PPRV. Therefore, Newcastle disease virus (NDV) with small RNA inserts based on RPV or PPRV sequences were generated and used as positive control material. Recombinant NDVs (recNDVs) were differentially detected by previously established PCR diagnostics for RPV or PPRV. Both recNDVs contain a second PCR target showing that additional targets in NDV are feasible and would increase the diagnostic sensitivity by use of two PCR assays. RecNDV with small PCR targets is not classified as RPV or PPRV containing material, and can be used to mimic RPV or PPRV. Using these recNDVs as virus positive material contributes to the ambition of worldwide eradication, while qualified PCR diagnostics for these OIE-listed diseases remains operational.
Original languageEnglish
Pages (from-to)50-53
JournalJournal of Virological Methods
Volume259
DOIs
Publication statusPublished - 12 Jun 2018

Fingerprint

Peste-des-Petits-Ruminants
Rinderpest
Newcastle disease virus
Polymerase Chain Reaction
Reverse Transcription
RNA
Viruses

Cite this

@article{2a5c7fc0141c4084a9249c3b89407108,
title = "Recombinant Newcastle disease viruses with targets for PCR diagnostics for rinderpest and peste des petits ruminants",
abstract = "Since February 1st 2011, rinderpest (RP) has been officially declared eradicated worldwide. National authorities have been requested to destroy all their RP related materials. Nonetheless, their national reference laboratories performing real time reverse transcription polymerase chain reaction assays (PCR diagnostics) need RP positive control samples, since some countries still prefer to maintain diagnostic capability for RP for several reasons. In the future, a similar situation will arise for peste des petits ruminants (PPR) as the ambition has been expressed to eradicate PPR. Anticipating on this, we intended to perform qualified PCR diagnostics without use of infectious RPV or PPRV. Therefore, Newcastle disease virus (NDV) with small RNA inserts based on RPV or PPRV sequences were generated and used as positive control material. Recombinant NDVs (recNDVs) were differentially detected by previously established PCR diagnostics for RPV or PPRV. Both recNDVs contain a second PCR target showing that additional targets in NDV are feasible and would increase the diagnostic sensitivity by use of two PCR assays. RecNDV with small PCR targets is not classified as RPV or PPRV containing material, and can be used to mimic RPV or PPRV. Using these recNDVs as virus positive material contributes to the ambition of worldwide eradication, while qualified PCR diagnostics for these OIE-listed diseases remains operational.",
author = "{van Rijn}, P.A. and J. Boonstra and {van Gennip}, H.G.P.",
year = "2018",
month = "6",
day = "12",
doi = "10.1016/j.jviromet.2018.06.007",
language = "English",
volume = "259",
pages = "50--53",
journal = "Journal of Virological Methods",
issn = "0166-0934",
publisher = "Elsevier",

}

Recombinant Newcastle disease viruses with targets for PCR diagnostics for rinderpest and peste des petits ruminants. / van Rijn, P.A.; Boonstra, J.; van Gennip, H.G.P.

In: Journal of Virological Methods, Vol. 259, 12.06.2018, p. 50-53.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Recombinant Newcastle disease viruses with targets for PCR diagnostics for rinderpest and peste des petits ruminants

AU - van Rijn, P.A.

AU - Boonstra, J.

AU - van Gennip, H.G.P.

PY - 2018/6/12

Y1 - 2018/6/12

N2 - Since February 1st 2011, rinderpest (RP) has been officially declared eradicated worldwide. National authorities have been requested to destroy all their RP related materials. Nonetheless, their national reference laboratories performing real time reverse transcription polymerase chain reaction assays (PCR diagnostics) need RP positive control samples, since some countries still prefer to maintain diagnostic capability for RP for several reasons. In the future, a similar situation will arise for peste des petits ruminants (PPR) as the ambition has been expressed to eradicate PPR. Anticipating on this, we intended to perform qualified PCR diagnostics without use of infectious RPV or PPRV. Therefore, Newcastle disease virus (NDV) with small RNA inserts based on RPV or PPRV sequences were generated and used as positive control material. Recombinant NDVs (recNDVs) were differentially detected by previously established PCR diagnostics for RPV or PPRV. Both recNDVs contain a second PCR target showing that additional targets in NDV are feasible and would increase the diagnostic sensitivity by use of two PCR assays. RecNDV with small PCR targets is not classified as RPV or PPRV containing material, and can be used to mimic RPV or PPRV. Using these recNDVs as virus positive material contributes to the ambition of worldwide eradication, while qualified PCR diagnostics for these OIE-listed diseases remains operational.

AB - Since February 1st 2011, rinderpest (RP) has been officially declared eradicated worldwide. National authorities have been requested to destroy all their RP related materials. Nonetheless, their national reference laboratories performing real time reverse transcription polymerase chain reaction assays (PCR diagnostics) need RP positive control samples, since some countries still prefer to maintain diagnostic capability for RP for several reasons. In the future, a similar situation will arise for peste des petits ruminants (PPR) as the ambition has been expressed to eradicate PPR. Anticipating on this, we intended to perform qualified PCR diagnostics without use of infectious RPV or PPRV. Therefore, Newcastle disease virus (NDV) with small RNA inserts based on RPV or PPRV sequences were generated and used as positive control material. Recombinant NDVs (recNDVs) were differentially detected by previously established PCR diagnostics for RPV or PPRV. Both recNDVs contain a second PCR target showing that additional targets in NDV are feasible and would increase the diagnostic sensitivity by use of two PCR assays. RecNDV with small PCR targets is not classified as RPV or PPRV containing material, and can be used to mimic RPV or PPRV. Using these recNDVs as virus positive material contributes to the ambition of worldwide eradication, while qualified PCR diagnostics for these OIE-listed diseases remains operational.

U2 - 10.1016/j.jviromet.2018.06.007

DO - 10.1016/j.jviromet.2018.06.007

M3 - Article

VL - 259

SP - 50

EP - 53

JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

ER -