Recombinant interleukin-5 induces in vivo airway hyperresponsiveness to histamine in guinea pigs

A.J.M. van Oosterhout, I. van Ark, G. Hofman, H.F.J. Savelkoul, F.P. Nijkamp

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Abstract

Interleukin-5-producing CV-1 cells were encapsulated in alginate and injected i.p. in guinea pigs (4 x 106/animal). These cells produced approximately 8 ng interleukin-5 per 4 x 106 cells per day. Airway hyperresponsiveness to histamine in vivo was observed 3 and 7 days after administration. The increase in lung resistance after intravenous administration of histamine to guinea pigs was significantly potentiated, by approximately 70 to 90% in interleukin-5-treated animals. In animals treated with antibody to interleukin-5, the administration of interleukin-5-producing CV-1 cells did not induce hyperresponsiveness. The percentage of eosinophils in broncho-alveolar lavage fluid was increased by 100% at 7 days but not a 3 days after administration of interleukin-5-producing CV-1 cells. Antibody to interleukin-5 prevented the broncho-alveolar lavage eosunophilia at 7 days after interleukin-5 administration. It can be concluded that interleukin-5 induces broncho-alveolar lavage eosinophilia and airway hyperresponsiveness and that these phenomena do not occur simultaneously. These data suggest a role for interleukin-5 in the development of airway hyperresponsiveness in bronchial asthma.
Original languageEnglish
Pages (from-to)379-383
JournalEuropean Journal of Pharmacology
Volume236
Issue number3
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Interleukin-5
Histamine
Guinea Pigs
Therapeutic Irrigation
Antibodies
Bronchoalveolar Lavage Fluid
Eosinophilia
Eosinophils
Intravenous Administration
Asthma
Lung

Cite this

van Oosterhout, A.J.M. ; van Ark, I. ; Hofman, G. ; Savelkoul, H.F.J. ; Nijkamp, F.P. / Recombinant interleukin-5 induces in vivo airway hyperresponsiveness to histamine in guinea pigs. In: European Journal of Pharmacology. 1993 ; Vol. 236, No. 3. pp. 379-383.
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abstract = "Interleukin-5-producing CV-1 cells were encapsulated in alginate and injected i.p. in guinea pigs (4 x 106/animal). These cells produced approximately 8 ng interleukin-5 per 4 x 106 cells per day. Airway hyperresponsiveness to histamine in vivo was observed 3 and 7 days after administration. The increase in lung resistance after intravenous administration of histamine to guinea pigs was significantly potentiated, by approximately 70 to 90{\%} in interleukin-5-treated animals. In animals treated with antibody to interleukin-5, the administration of interleukin-5-producing CV-1 cells did not induce hyperresponsiveness. The percentage of eosinophils in broncho-alveolar lavage fluid was increased by 100{\%} at 7 days but not a 3 days after administration of interleukin-5-producing CV-1 cells. Antibody to interleukin-5 prevented the broncho-alveolar lavage eosunophilia at 7 days after interleukin-5 administration. It can be concluded that interleukin-5 induces broncho-alveolar lavage eosinophilia and airway hyperresponsiveness and that these phenomena do not occur simultaneously. These data suggest a role for interleukin-5 in the development of airway hyperresponsiveness in bronchial asthma.",
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Recombinant interleukin-5 induces in vivo airway hyperresponsiveness to histamine in guinea pigs. / van Oosterhout, A.J.M.; van Ark, I.; Hofman, G.; Savelkoul, H.F.J.; Nijkamp, F.P.

In: European Journal of Pharmacology, Vol. 236, No. 3, 1993, p. 379-383.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Recombinant interleukin-5 induces in vivo airway hyperresponsiveness to histamine in guinea pigs

AU - van Oosterhout, A.J.M.

AU - van Ark, I.

AU - Hofman, G.

AU - Savelkoul, H.F.J.

AU - Nijkamp, F.P.

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N2 - Interleukin-5-producing CV-1 cells were encapsulated in alginate and injected i.p. in guinea pigs (4 x 106/animal). These cells produced approximately 8 ng interleukin-5 per 4 x 106 cells per day. Airway hyperresponsiveness to histamine in vivo was observed 3 and 7 days after administration. The increase in lung resistance after intravenous administration of histamine to guinea pigs was significantly potentiated, by approximately 70 to 90% in interleukin-5-treated animals. In animals treated with antibody to interleukin-5, the administration of interleukin-5-producing CV-1 cells did not induce hyperresponsiveness. The percentage of eosinophils in broncho-alveolar lavage fluid was increased by 100% at 7 days but not a 3 days after administration of interleukin-5-producing CV-1 cells. Antibody to interleukin-5 prevented the broncho-alveolar lavage eosunophilia at 7 days after interleukin-5 administration. It can be concluded that interleukin-5 induces broncho-alveolar lavage eosinophilia and airway hyperresponsiveness and that these phenomena do not occur simultaneously. These data suggest a role for interleukin-5 in the development of airway hyperresponsiveness in bronchial asthma.

AB - Interleukin-5-producing CV-1 cells were encapsulated in alginate and injected i.p. in guinea pigs (4 x 106/animal). These cells produced approximately 8 ng interleukin-5 per 4 x 106 cells per day. Airway hyperresponsiveness to histamine in vivo was observed 3 and 7 days after administration. The increase in lung resistance after intravenous administration of histamine to guinea pigs was significantly potentiated, by approximately 70 to 90% in interleukin-5-treated animals. In animals treated with antibody to interleukin-5, the administration of interleukin-5-producing CV-1 cells did not induce hyperresponsiveness. The percentage of eosinophils in broncho-alveolar lavage fluid was increased by 100% at 7 days but not a 3 days after administration of interleukin-5-producing CV-1 cells. Antibody to interleukin-5 prevented the broncho-alveolar lavage eosunophilia at 7 days after interleukin-5 administration. It can be concluded that interleukin-5 induces broncho-alveolar lavage eosinophilia and airway hyperresponsiveness and that these phenomena do not occur simultaneously. These data suggest a role for interleukin-5 in the development of airway hyperresponsiveness in bronchial asthma.

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