Recombinant cell bioassays for the detection of (gluco) corticosteroids and endocrine-disrupting potencies of several enviromental PCB contaminants

T.F.H. Bovee, J.R. Helsdingen, A.R.M. Hamers, B.A. Brouwer, M.W.F. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

32 Citations (Scopus)

Abstract

Sensitive and robust bioassays for glucocorticoids are very useful for the pharmaceutical industry, environmental scientists and veterinary control. Here, a recombinant yeast cell was constructed that expresses the human glucocorticoid receptor alpha and a green fluorescent reporter protein in response to glucocorticoids. Both the receptor construct and the reporter construct were stably integrated into the yeast genome. The correct and specific functioning of this yeast glucocorticoid bioassay was studied by exposures to cortisol and other related compounds and critically compared to a GR-CALUX bioassay based on a human bone cell. Although less sensitive, the new yeast glucocorticoid bioassay showed sensitivity towards all (gluco)corticoids tested, with the following order in relative potencies: budesonide >> corticosterone > dexamethasone > cortisol = betamethasone > prednisolone > aldosterone. Hormone representatives for other hormone nuclear receptors, like 17ß-estradiol for the oestrogen receptor, 5a-dihydrotestosterone for the androgen receptor and progesterone for the progesterone receptor, showed no clear agonistic responses, whilst some polychlorinated biphenyls were clearly able to interfere with the GR activity.
Original languageEnglish
Pages (from-to)873-882
JournalAnalytical and Bioanalytical Chemistry
Volume401
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • human glucocorticoid-receptor
  • green fluorescent protein
  • in-vitro
  • androgen receptor
  • growth promoters
  • beta-isoform
  • progesterone
  • expression
  • gene
  • transcription

Fingerprint Dive into the research topics of 'Recombinant cell bioassays for the detection of (gluco) corticosteroids and endocrine-disrupting potencies of several enviromental PCB contaminants'. Together they form a unique fingerprint.

Cite this