Reciprocal cybrids reveal how organellar genomes affect plant phenotypes

Pádraic J. Flood*, Tom P.J.M. Theeuwen, Korbinian Schneeberger, Paul Keizer, Willem Kruijer, Edouard Severing, Evangelos Kouklas, Jos A. Hageman, Raúl Wijfjes, Vanesa Calvo-Baltanas, Frank F.M. Becker, Sabine K. Schnabel, Leo A.J. Willems, Wilco Ligterink, Jeroen Van Arkel, Roland Mumm, José M. Gualberto, Linda Savage, David M. Kramer, Joost J.B. KeurentjesFred Van Eeuwijk, Maarten Koornneef, Jeremy Harbinson, Mark G.M. Aarts, Erik Wijnker

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Assessment of the impact of variation in chloroplast and mitochondrial DNA (collectively termed the plasmotype) on plant phenotypes is challenging due to the difficulty in separating their effect from nuclear-derived variation (the nucleotype). Haploid-inducer lines can be used as efficient plasmotype donors to generate new plasmotype–nucleotype combinations (cybrids)1. We generated a panel comprising all possible cybrids of seven Arabidopsis thaliana accessions and extensively phenotyped these lines for 1,859 phenotypes under both stable and fluctuating conditions. We show that natural variation in the plasmotype results in both additive and epistatic effects across all phenotypic categories. Plasmotypes that induce more additive phenotypic changes also cause more epistatic effects, suggesting a possible common basis for both additive and epistatic effects. On average, epistatic interactions explained twice as much of the variance in phenotypes as additive plasmotype effects. The impact of plasmotypic variation was also more pronounced under fluctuating and stressful environmental conditions. Thus, the phenotypic impact of variation in plasmotypes is the outcome of multi-level nucleotype–plasmotype–environment interactions and, as such, the plasmotype is likely to serve as a reservoir of variation that is predominantly exposed under certain conditions. The production of cybrids using haploid inducers is a rapid and precise method for assessment of the phenotypic effects of natural variation in organellar genomes. It will facilitate efficient screening of unique nucleotype–plasmotype combinations to both improve our understanding of natural variation in these combinations and identify favourable combinations to enhance plant performance.
Original languageEnglish
Pages (from-to)13-21
JournalNature Plants
Volume6
Issue number1
DOIs
Publication statusPublished - 13 Jan 2020

Fingerprint Dive into the research topics of 'Reciprocal cybrids reveal how organellar genomes affect plant phenotypes'. Together they form a unique fingerprint.

  • Datasets

    Reciprocal cybrids reveal how organellar genomes affect plant phenotypes - RNA Sequencing

    Flood, P. (Creator), Theeuwen, T. (Creator), Schneeberger, K. (Creator), Keizer, P. (Creator), Kruijer, W. (Creator), Severing, E. (Creator), Kouklas, E. (Creator), Hageman, J. (Creator), Wijfjes, R. (Creator), Calvo Baltanas, V. (Creator), Becker, F. (Creator), Schnabel, S. (Creator), Willems, L. (Creator), Ligterink, W. (Creator), van Arkel, J. (Creator), Mumm, R. (Creator), Gualberto, J. M. (Creator), Savage, L. (Creator), Kramer, D. M. (Creator), Keurentjes, J. (Creator), van Eeuwijk, F. (Creator), Koornneef, M. (Creator), Harbinson, J. (Creator), Aarts, M. (Creator) & Wijnker, E. (Creator), Wageningen University, 12 Nov 2019

    Dataset

    Reciprocal cybrids reveal how organellar genomes affect plant phenotypes

    Theeuwen, T. (Creator), Flood, P. (Creator), Schneeberger, K. (Creator), Kruijer, W. (Creator), Severing, E. (Creator), Kouklas, E. (Creator), Hageman, J. (Creator), Wijfjes, R. (Creator), Calvo Baltanas, V. (Creator), Becker, F. (Creator), Schnabel, S. (Creator), Willems, L. (Creator), Ligterink, W. (Creator), van Arkel, J. (Creator), Mumm, R. (Creator), Gualberto, J. M. (Creator), Savage, L. (Creator), Kramer, D. M. (Creator), Keurentjes, J. (Creator), van Eeuwijk, F. (Creator), Koornneef, M. (Creator), Harbinson, J. (Creator), Aarts, M. (Creator) & Wijnker, E. (Creator), Wageningen University & Research, 19 Dec 2019

    Dataset

    Reciprocal cybrids reveal how organellar genomes affect plant phenotypes - Whole Genome Sequencing

    Flood, P. (Creator), Theeuwen, T. (Creator), Schneeberger, K. (Creator), Keizer, P. (Creator), Kruijer, W. (Creator), Severing, E. (Creator), Kouklas, E. (Creator), Hageman, J. (Creator), Wijfjes, R. (Creator), Calvo Baltanas, V. (Creator), Becker, F. (Creator), Schnabel, S. (Creator), Willems, L. (Creator), Ligterink, W. (Creator), van Arkel, J. (Creator), Mumm, R. (Creator), Gualberto, J. M. (Creator), Savage, L. (Creator), Kramer, D. M. (Creator), Keurentjes, J. (Creator), van Eeuwijk, F. (Creator), Koornneef, M. (Creator), Harbinson, J. (Creator), Aarts, M. (Creator) & Wijnker, E. (Creator), Wageningen University, 11 Nov 2019

    Dataset

    Press / Media

    Plants could capture more sun

    Tom Theeuwen, Erik Wijnker & Jeremy Harbinson

    13/02/2014/02/20

    2 Media contributions

    Press/Media: Research

    Cite this