Receptor kinase module targets PIN-dependent auxin transport during canalization

Jakub Hajný; Tomáš Prát; Nikola Rydza; Lesia Rodriguez; Shutang Tan; Inge Verstraeten; David Domjan; Ewa Mazur; Elwira Smakowska-Luzan; Wouter Smet; Eliana Mor; Jonah Nolf; Bao Jun Yang; Wim Grunewald; Gergely Molnár; Youssef Belkhadir; Bert De Rybel; Jiří Friml

doi:10.1126/science.aba3178

Receptor kinase module targets PIN-dependent auxin transport during canalization

Jakub Hajný, Tomáš Prát, Nikola Rydza, Lesia Rodriguez, Shutang Tan, Inge Verstraeten, David Domjan, Ewa Mazur, Elwira Smakowska-Luzan, Wouter Smet, Eliana Mor, Jonah Nolf, Bao Jun Yang, Wim Grunewald, Gergely Molnár, Youssef Belkhadir, Bert De Rybel, Jiří Friml^*

^*Corresponding author for this work

Biochemistry

Research output: Contribution to journal › Article › Academic › peer-review

1 Citation (Scopus)

Abstract

Spontaneously arising channels that transport the phytohormone auxin provide positional cues for self-organizing aspects of plant development such as flexible vasculature regeneration or its patterning during leaf venation. The auxin canalization hypothesis proposes a feedback between auxin signaling and transport as the underlying mechanism, but molecular players await discovery. We identified part of the machinery that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like kinase) interact with and phosphorylate PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization, which macroscopically manifests as defects in leaf venation and vasculature regeneration after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback that coordinates polarization of individual cells during auxin canalization.

Original language	English
Pages (from-to)	550-557
Number of pages	8
Journal	Science (New York, N.Y.)
Volume	370
Issue number	6516
DOIs	https://doi.org/10.1126/science.aba3178
Publication status	Published - 30 Oct 2020

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Hajný, J., Prát, T., Rydza, N., Rodriguez, L., Tan, S., Verstraeten, I., Domjan, D., Mazur, E., Smakowska-Luzan, E., Smet, W., Mor, E., Nolf, J., Yang, B. J., Grunewald, W., Molnár, G., Belkhadir, Y., De Rybel, B., & Friml, J. (2020). Receptor kinase module targets PIN-dependent auxin transport during canalization. Science (New York, N.Y.), 370(6516), 550-557. https://doi.org/10.1126/science.aba3178

Hajný, Jakub ; Prát, Tomáš ; Rydza, Nikola ; Rodriguez, Lesia ; Tan, Shutang ; Verstraeten, Inge ; Domjan, David ; Mazur, Ewa ; Smakowska-Luzan, Elwira ; Smet, Wouter ; Mor, Eliana ; Nolf, Jonah ; Yang, Bao Jun ; Grunewald, Wim ; Molnár, Gergely ; Belkhadir, Youssef ; De Rybel, Bert ; Friml, Jiří. / Receptor kinase module targets PIN-dependent auxin transport during canalization. In: Science (New York, N.Y.). 2020 ; Vol. 370, No. 6516. pp. 550-557.

@article{5b7737be80da48b4b940046985ccbcb3,

title = "Receptor kinase module targets PIN-dependent auxin transport during canalization",

abstract = "Spontaneously arising channels that transport the phytohormone auxin provide positional cues for self-organizing aspects of plant development such as flexible vasculature regeneration or its patterning during leaf venation. The auxin canalization hypothesis proposes a feedback between auxin signaling and transport as the underlying mechanism, but molecular players await discovery. We identified part of the machinery that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like kinase) interact with and phosphorylate PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization, which macroscopically manifests as defects in leaf venation and vasculature regeneration after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback that coordinates polarization of individual cells during auxin canalization.",

author = "Jakub Hajn{\'y} and Tom{\'a}{\v s} Pr{\'a}t and Nikola Rydza and Lesia Rodriguez and Shutang Tan and Inge Verstraeten and David Domjan and Ewa Mazur and Elwira Smakowska-Luzan and Wouter Smet and Eliana Mor and Jonah Nolf and Yang, {Bao Jun} and Wim Grunewald and Gergely Moln{\'a}r and Youssef Belkhadir and {De Rybel}, Bert and Ji{\v r}{\'i} Friml",

year = "2020",

month = oct,

day = "30",

doi = "10.1126/science.aba3178",

language = "English",

volume = "370",

pages = "550--557",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6516",

}

Hajný, J, Prát, T, Rydza, N, Rodriguez, L, Tan, S, Verstraeten, I, Domjan, D, Mazur, E, Smakowska-Luzan, E, Smet, W, Mor, E, Nolf, J, Yang, BJ, Grunewald, W, Molnár, G, Belkhadir, Y, De Rybel, B & Friml, J 2020, 'Receptor kinase module targets PIN-dependent auxin transport during canalization', Science (New York, N.Y.), vol. 370, no. 6516, pp. 550-557. https://doi.org/10.1126/science.aba3178

Receptor kinase module targets PIN-dependent auxin transport during canalization. / Hajný, Jakub; Prát, Tomáš; Rydza, Nikola; Rodriguez, Lesia; Tan, Shutang; Verstraeten, Inge; Domjan, David; Mazur, Ewa; Smakowska-Luzan, Elwira; Smet, Wouter; Mor, Eliana; Nolf, Jonah; Yang, Bao Jun; Grunewald, Wim; Molnár, Gergely; Belkhadir, Youssef; De Rybel, Bert; Friml, Jiří.

In: Science (New York, N.Y.), Vol. 370, No. 6516, 30.10.2020, p. 550-557.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Receptor kinase module targets PIN-dependent auxin transport during canalization

AU - Hajný, Jakub

AU - Prát, Tomáš

AU - Rydza, Nikola

AU - Rodriguez, Lesia

AU - Tan, Shutang

AU - Verstraeten, Inge

AU - Domjan, David

AU - Mazur, Ewa

AU - Smakowska-Luzan, Elwira

AU - Smet, Wouter

AU - Mor, Eliana

AU - Nolf, Jonah

AU - Yang, Bao Jun

AU - Grunewald, Wim

AU - Molnár, Gergely

AU - Belkhadir, Youssef

AU - De Rybel, Bert

AU - Friml, Jiří

PY - 2020/10/30

Y1 - 2020/10/30

N2 - Spontaneously arising channels that transport the phytohormone auxin provide positional cues for self-organizing aspects of plant development such as flexible vasculature regeneration or its patterning during leaf venation. The auxin canalization hypothesis proposes a feedback between auxin signaling and transport as the underlying mechanism, but molecular players await discovery. We identified part of the machinery that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like kinase) interact with and phosphorylate PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization, which macroscopically manifests as defects in leaf venation and vasculature regeneration after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback that coordinates polarization of individual cells during auxin canalization.

AB - Spontaneously arising channels that transport the phytohormone auxin provide positional cues for self-organizing aspects of plant development such as flexible vasculature regeneration or its patterning during leaf venation. The auxin canalization hypothesis proposes a feedback between auxin signaling and transport as the underlying mechanism, but molecular players await discovery. We identified part of the machinery that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like kinase) interact with and phosphorylate PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization, which macroscopically manifests as defects in leaf venation and vasculature regeneration after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback that coordinates polarization of individual cells during auxin canalization.

U2 - 10.1126/science.aba3178

DO - 10.1126/science.aba3178

M3 - Article

C2 - 33122378

AN - SCOPUS:85094936069

VL - 370

SP - 550

EP - 557

JO - Science

JF - Science

SN - 0036-8075

IS - 6516

ER -