Reassessing the role of phospholipase D in the Arabidopsis wounding response

Bastiaan O.R. Bargmann, Ana M. Laxalt, Bas Ter Riet, Christa Testerink, Emmanuelle Merquiol, Alina Mosblech, Antonio Leon-Reyes, Corné M.J. Pieterse, Michel A. Haring, Ingo Heilmann, Dorothea Bartels, Teun Munnik*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

48 Citations (Scopus)

Abstract

Plants respond to wounding by means of a multitude of reactions, with the purpose of stifling herbivore assault. Phospholipase D (PLD) has previously been implicated in the wounding response. Arabidopsis (Arabidopsis thaliana) AtPLDα1 has been proposed to be activated in intact cells, and the phosphatidic acid (PA) it produces to serve as a precursor for jasmonic acid (JA) synthesis and to be required for wounding-induced gene expression. Independently, PLD activity has been reported to have a bearing on wounding-induced MAPK activation. However, which PLD isoforms are activated, where this activity takes place (in the wounded or non-wounded cells) and what exactly the consequences are is a question that has not been comprehensively addressed. Here, we show that PLD activity during the wounding response is restricted to the ruptured cells using 32Pi-labelled phospholipid analyses of Arabidopsis pld knock-out mutants and PLD-silenced tomato cell-suspension cultures. pldα1 knock-out lines have reduced wounding-induced PA production, and the remainder is completely eliminated in a pldα1/δ double knock-out line. Surprisingly, wounding-induced protein kinase activation, AtLOX2 gene expression and JA biosynthesis were not affected in these knock-out lines. Moreover, larvae of the Cabbage White butterfly (Pieris rapae) grew equally well on wild-type and the pld knock-out mutants.

Original languageEnglish
Pages (from-to)837-850
Number of pages14
JournalPlant, Cell and Environment
Volume32
Issue number7
DOIs
Publication statusPublished - Jul 2009
Externally publishedYes

Keywords

  • Jasmonic acid
  • Phosphatidic acid
  • PLD

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    Bargmann, B. O. R., Laxalt, A. M., Riet, B. T., Testerink, C., Merquiol, E., Mosblech, A., ... Munnik, T. (2009). Reassessing the role of phospholipase D in the Arabidopsis wounding response. Plant, Cell and Environment, 32(7), 837-850. https://doi.org/10.1111/j.1365-3040.2009.01962.x