Real-Time PCRs and Fingerprinting assays for the Detection and Characterization of Salmonella Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of Salmonella, Escherichia coli, Shigella and Proteus

C.F. Amar, C.A. Arnold, A. Bankier, P.H. Dear, B. Guerra, K.L. Hopkins, E. Liebana, D.J. Mevius, E.J. Threllfall

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

Salmonella Genomic Island-1 (SGI-1) harbors a cluster of genes encoding multidrug resistance (MDR). SGI-1 is horizontally transmissible and is therefore of significant public health concern. This study presents two novel realtime PCRs detecting three SGI-1 protein-coding genes and a SGI-1 fingerprinting assay. These assays were applied to 445 European enterobacterial isolates. Results from real-time PCRs were comparable to those obtained from gelbased PCRs used for the detection of SGI-1, but were rapid to perform and suitable for large-scale screening. Furthermore, real-time PCRs also detected SGI-1 even when only part of the island was present in bacterial isolates. No trace of SGI-1 was detected in isolates other than Salmonella enterica. The fingerprints showed that regions of SGI-1 outside the MDR region exhibited genomic variations between isolates. In conclusion, the realtime PCRs described here are suitable for the detection of SGI-1 in bacterial isolates. Further studies are necessary to elucidate divergence in its non-MDR region.
Original languageEnglish
Pages (from-to)79-92
JournalMicrobial Drug Resistance-Mechanisms Epidemiology and Disease
Volume14
Issue number2
DOIs
Publication statusPublished - 2008

Keywords

  • multiple-antibiotic-resistance
  • serovar typhimurium dt104
  • serotype paratyphi-b
  • genomic island sgi1
  • enterica serovars
  • drug-resistance
  • gene clusters
  • variant
  • region
  • integrons

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