Real-Time PCRs and Fingerprinting assays for the Detection and Characterization of Salmonella Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of Salmonella, Escherichia coli, Shigella and Proteus

C.F. Amar; C.A. Arnold; A. Bankier; P.H. Dear; B. Guerra; K.L. Hopkins; E. Liebana; D.J. Mevius; E.J. Threllfall

doi:10.1089/mdr.2008.0812

Real-Time PCRs and Fingerprinting assays for the Detection and Characterization of Salmonella Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of Salmonella, Escherichia coli, Shigella and Proteus

C.F. Amar, C.A. Arnold, A. Bankier, P.H. Dear, B. Guerra, K.L. Hopkins, E. Liebana, D.J. Mevius, E.J. Threllfall

Department of Social Sciences

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

Salmonella Genomic Island-1 (SGI-1) harbors a cluster of genes encoding multidrug resistance (MDR). SGI-1 is horizontally transmissible and is therefore of significant public health concern. This study presents two novel realtime PCRs detecting three SGI-1 protein-coding genes and a SGI-1 fingerprinting assay. These assays were applied to 445 European enterobacterial isolates. Results from real-time PCRs were comparable to those obtained from gelbased PCRs used for the detection of SGI-1, but were rapid to perform and suitable for large-scale screening. Furthermore, real-time PCRs also detected SGI-1 even when only part of the island was present in bacterial isolates. No trace of SGI-1 was detected in isolates other than Salmonella enterica. The fingerprints showed that regions of SGI-1 outside the MDR region exhibited genomic variations between isolates. In conclusion, the realtime PCRs described here are suitable for the detection of SGI-1 in bacterial isolates. Further studies are necessary to elucidate divergence in its non-MDR region.

Original language	English
Pages (from-to)	79-92
Journal	Microbial Drug Resistance-Mechanisms Epidemiology and Disease
Volume	14
Issue number	2
DOIs	https://doi.org/10.1089/mdr.2008.0812
Publication status	Published - 2008

Keywords

multiple-antibiotic-resistance
serovar typhimurium dt104
serotype paratyphi-b
genomic island sgi1
enterica serovars
drug-resistance
gene clusters
variant
region
integrons

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Amar, C. F., Arnold, C. A., Bankier, A., Dear, P. H., Guerra, B., Hopkins, K. L., Liebana, E., Mevius, D. J., & Threllfall, E. J. (2008). Real-Time PCRs and Fingerprinting assays for the Detection and Characterization of Salmonella Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of Salmonella, Escherichia coli, Shigella and Proteus. Microbial Drug Resistance-Mechanisms Epidemiology and Disease, 14(2), 79-92. https://doi.org/10.1089/mdr.2008.0812

Amar, C.F. ; Arnold, C.A. ; Bankier, A. et al. / Real-Time PCRs and Fingerprinting assays for the Detection and Characterization of Salmonella Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of Salmonella, Escherichia coli, Shigella and Proteus. In: Microbial Drug Resistance-Mechanisms Epidemiology and Disease. 2008 ; Vol. 14, No. 2. pp. 79-92.

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title = "Real-Time PCRs and Fingerprinting assays for the Detection and Characterization of Salmonella Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of Salmonella, Escherichia coli, Shigella and Proteus",

abstract = "Salmonella Genomic Island-1 (SGI-1) harbors a cluster of genes encoding multidrug resistance (MDR). SGI-1 is horizontally transmissible and is therefore of significant public health concern. This study presents two novel realtime PCRs detecting three SGI-1 protein-coding genes and a SGI-1 fingerprinting assay. These assays were applied to 445 European enterobacterial isolates. Results from real-time PCRs were comparable to those obtained from gelbased PCRs used for the detection of SGI-1, but were rapid to perform and suitable for large-scale screening. Furthermore, real-time PCRs also detected SGI-1 even when only part of the island was present in bacterial isolates. No trace of SGI-1 was detected in isolates other than Salmonella enterica. The fingerprints showed that regions of SGI-1 outside the MDR region exhibited genomic variations between isolates. In conclusion, the realtime PCRs described here are suitable for the detection of SGI-1 in bacterial isolates. Further studies are necessary to elucidate divergence in its non-MDR region.",

keywords = "multiple-antibiotic-resistance, serovar typhimurium dt104, serotype paratyphi-b, genomic island sgi1, enterica serovars, drug-resistance, gene clusters, variant, region, integrons",

author = "C.F. Amar and C.A. Arnold and A. Bankier and P.H. Dear and B. Guerra and K.L. Hopkins and E. Liebana and D.J. Mevius and E.J. Threllfall",

year = "2008",

doi = "10.1089/mdr.2008.0812",

language = "English",

volume = "14",

pages = "79--92",

journal = "Microbial Drug Resistance-Mechanisms Epidemiology and Disease",

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Amar, CF, Arnold, CA, Bankier, A, Dear, PH, Guerra, B, Hopkins, KL, Liebana, E, Mevius, DJ & Threllfall, EJ 2008, 'Real-Time PCRs and Fingerprinting assays for the Detection and Characterization of Salmonella Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of Salmonella, Escherichia coli, Shigella and Proteus', Microbial Drug Resistance-Mechanisms Epidemiology and Disease, vol. 14, no. 2, pp. 79-92. https://doi.org/10.1089/mdr.2008.0812

Real-Time PCRs and Fingerprinting assays for the Detection and Characterization of Salmonella Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of Salmonella, Escherichia coli, Shigella and Proteus. / Amar, C.F.; Arnold, C.A.; Bankier, A. et al.
In: Microbial Drug Resistance-Mechanisms Epidemiology and Disease, Vol. 14, No. 2, 2008, p. 79-92.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Real-Time PCRs and Fingerprinting assays for the Detection and Characterization of Salmonella Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of Salmonella, Escherichia coli, Shigella and Proteus

AU - Amar, C.F.

AU - Arnold, C.A.

AU - Bankier, A.

AU - Dear, P.H.

AU - Guerra, B.

AU - Hopkins, K.L.

AU - Liebana, E.

AU - Mevius, D.J.

AU - Threllfall, E.J.

PY - 2008

Y1 - 2008

N2 - Salmonella Genomic Island-1 (SGI-1) harbors a cluster of genes encoding multidrug resistance (MDR). SGI-1 is horizontally transmissible and is therefore of significant public health concern. This study presents two novel realtime PCRs detecting three SGI-1 protein-coding genes and a SGI-1 fingerprinting assay. These assays were applied to 445 European enterobacterial isolates. Results from real-time PCRs were comparable to those obtained from gelbased PCRs used for the detection of SGI-1, but were rapid to perform and suitable for large-scale screening. Furthermore, real-time PCRs also detected SGI-1 even when only part of the island was present in bacterial isolates. No trace of SGI-1 was detected in isolates other than Salmonella enterica. The fingerprints showed that regions of SGI-1 outside the MDR region exhibited genomic variations between isolates. In conclusion, the realtime PCRs described here are suitable for the detection of SGI-1 in bacterial isolates. Further studies are necessary to elucidate divergence in its non-MDR region.

AB - Salmonella Genomic Island-1 (SGI-1) harbors a cluster of genes encoding multidrug resistance (MDR). SGI-1 is horizontally transmissible and is therefore of significant public health concern. This study presents two novel realtime PCRs detecting three SGI-1 protein-coding genes and a SGI-1 fingerprinting assay. These assays were applied to 445 European enterobacterial isolates. Results from real-time PCRs were comparable to those obtained from gelbased PCRs used for the detection of SGI-1, but were rapid to perform and suitable for large-scale screening. Furthermore, real-time PCRs also detected SGI-1 even when only part of the island was present in bacterial isolates. No trace of SGI-1 was detected in isolates other than Salmonella enterica. The fingerprints showed that regions of SGI-1 outside the MDR region exhibited genomic variations between isolates. In conclusion, the realtime PCRs described here are suitable for the detection of SGI-1 in bacterial isolates. Further studies are necessary to elucidate divergence in its non-MDR region.

KW - multiple-antibiotic-resistance

KW - serovar typhimurium dt104

KW - serotype paratyphi-b

KW - genomic island sgi1

KW - enterica serovars

KW - drug-resistance

KW - gene clusters

KW - variant

KW - region

KW - integrons

U2 - 10.1089/mdr.2008.0812

DO - 10.1089/mdr.2008.0812

M3 - Article

SN - 1076-6294

VL - 14

SP - 79

EP - 92

JO - Microbial Drug Resistance-Mechanisms Epidemiology and Disease

JF - Microbial Drug Resistance-Mechanisms Epidemiology and Disease

IS - 2

ER -

Amar CF, Arnold CA, Bankier A, Dear PH, Guerra B, Hopkins KL et al. Real-Time PCRs and Fingerprinting assays for the Detection and Characterization of Salmonella Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of Salmonella, Escherichia coli, Shigella and Proteus. Microbial Drug Resistance-Mechanisms Epidemiology and Disease. 2008;14(2):79-92. doi: 10.1089/mdr.2008.0812