Real-time PCR detection of ruminant DNA

L. Mendoza - Romero, E.L.C. Verkaar, P.H. Savelkoul, A. Catsburg, H.J.M. Aarts, J.B. Buntjer, J.A. Lenstra

Research output: Contribution to journalArticleAcademicpeer-review

44 Citations (Scopus)

Abstract

To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10␋ovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134°C for 3 instead of 20 min.
Original languageEnglish
Pages (from-to)550-554
JournalJournal of Food Protection
Volume67
Issue number3
Publication statusPublished - 2004

Keywords

  • bovine spongiform encephalopathy
  • mitochondrial-dna
  • bone meal
  • species identification
  • animal feedstuffs
  • satellite dna
  • evolution
  • cattle
  • transmission
  • sequences

Fingerprint Dive into the research topics of 'Real-time PCR detection of ruminant DNA'. Together they form a unique fingerprint.

  • Cite this

    Mendoza - Romero, L., Verkaar, E. L. C., Savelkoul, P. H., Catsburg, A., Aarts, H. J. M., Buntjer, J. B., & Lenstra, J. A. (2004). Real-time PCR detection of ruminant DNA. Journal of Food Protection, 67(3), 550-554. http://www.ingentaconnect.com/content/iafp/jfp/2004/00000067/00000003/art00019