Real-time PCR assay for the detection of Thrips palmi

L.F.F. Kox, H.E. van den Beld, C. Zijlstra, G. Vierbergen

    Research output: Contribution to journalArticleAcademicpeer-review

    20 Citations (Scopus)

    Abstract

    Since Thrips palmi became a regulated pest for most European countries, inspections at points of entry into Europe and monitoring in Europe have intensified not only for T. palmi but also for thrips as a whole. Morphological identification of thrips is performed on adults and to a lesser extent on second-stage larvae only, because no adequate identification keys for the separation of species based on the characteristics of eggs, first-stage larvae, pre-pupae or pupae are available. We have developed a real-time PCR assay based on TaqMan. A T. palmi-specific set of primers and probe were selected within the mitochondrial cytochrome oxidase I (COI) gene. The specificity of the assay was assessed using 15 specimens of Thrips palmi and 61 specimens of 23 other thrips species commonly occuring in Europe. All T. palmi specimens were detected, and no cross reactions with other thrips were observed. The method was tested on single larvae and adults and proved to be applicable for both those stages of T. palmi
    Original languageEnglish
    Pages (from-to)141-148
    JournalEPPO Bulletin
    Volume35
    Issue number1
    DOIs
    Publication statusPublished - 2005

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