Real-time PCR assay for the detection of Thrips palmi

L.F.F. Kox, H.E. van den Beld, C. Zijlstra, G. Vierbergen

    Research output: Contribution to journalArticleAcademicpeer-review

    16 Citations (Scopus)

    Abstract

    Since Thrips palmi became a regulated pest for most European countries, inspections at points of entry into Europe and monitoring in Europe have intensified not only for T. palmi but also for thrips as a whole. Morphological identification of thrips is performed on adults and to a lesser extent on second-stage larvae only, because no adequate identification keys for the separation of species based on the characteristics of eggs, first-stage larvae, pre-pupae or pupae are available. We have developed a real-time PCR assay based on TaqMan. A T. palmi-specific set of primers and probe were selected within the mitochondrial cytochrome oxidase I (COI) gene. The specificity of the assay was assessed using 15 specimens of Thrips palmi and 61 specimens of 23 other thrips species commonly occuring in Europe. All T. palmi specimens were detected, and no cross reactions with other thrips were observed. The method was tested on single larvae and adults and proved to be applicable for both those stages of T. palmi
    Original languageEnglish
    Pages (from-to)141-148
    JournalEPPO Bulletin
    Volume35
    Issue number1
    DOIs
    Publication statusPublished - 2005

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    Thrips palmi
    quantitative polymerase chain reaction
    Thysanoptera
    assays
    pupae
    larvae
    taxonomic keys
    egg quality
    cross reaction
    cytochrome-c oxidase
    pests
    monitoring

    Cite this

    Kox, L.F.F. ; van den Beld, H.E. ; Zijlstra, C. ; Vierbergen, G. / Real-time PCR assay for the detection of Thrips palmi. In: EPPO Bulletin. 2005 ; Vol. 35, No. 1. pp. 141-148.
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    abstract = "Since Thrips palmi became a regulated pest for most European countries, inspections at points of entry into Europe and monitoring in Europe have intensified not only for T. palmi but also for thrips as a whole. Morphological identification of thrips is performed on adults and to a lesser extent on second-stage larvae only, because no adequate identification keys for the separation of species based on the characteristics of eggs, first-stage larvae, pre-pupae or pupae are available. We have developed a real-time PCR assay based on TaqMan. A T. palmi-specific set of primers and probe were selected within the mitochondrial cytochrome oxidase I (COI) gene. The specificity of the assay was assessed using 15 specimens of Thrips palmi and 61 specimens of 23 other thrips species commonly occuring in Europe. All T. palmi specimens were detected, and no cross reactions with other thrips were observed. The method was tested on single larvae and adults and proved to be applicable for both those stages of T. palmi",
    author = "L.F.F. Kox and {van den Beld}, H.E. and C. Zijlstra and G. Vierbergen",
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    Real-time PCR assay for the detection of Thrips palmi. / Kox, L.F.F.; van den Beld, H.E.; Zijlstra, C.; Vierbergen, G.

    In: EPPO Bulletin, Vol. 35, No. 1, 2005, p. 141-148.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - Real-time PCR assay for the detection of Thrips palmi

    AU - Kox, L.F.F.

    AU - van den Beld, H.E.

    AU - Zijlstra, C.

    AU - Vierbergen, G.

    PY - 2005

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    AB - Since Thrips palmi became a regulated pest for most European countries, inspections at points of entry into Europe and monitoring in Europe have intensified not only for T. palmi but also for thrips as a whole. Morphological identification of thrips is performed on adults and to a lesser extent on second-stage larvae only, because no adequate identification keys for the separation of species based on the characteristics of eggs, first-stage larvae, pre-pupae or pupae are available. We have developed a real-time PCR assay based on TaqMan. A T. palmi-specific set of primers and probe were selected within the mitochondrial cytochrome oxidase I (COI) gene. The specificity of the assay was assessed using 15 specimens of Thrips palmi and 61 specimens of 23 other thrips species commonly occuring in Europe. All T. palmi specimens were detected, and no cross reactions with other thrips were observed. The method was tested on single larvae and adults and proved to be applicable for both those stages of T. palmi

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