Real-time PCR as a promising tool to monitor the epidemiology of Venturia pirina.

M.M. van Driel*, K. Kots*, M. Wenneker*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademicpeer-review

Abstract

Current control practices for pear scab (Venturia pirina) rely on prevention through orchard sanitation and extensive fungicide applications. Molecular quantification through a qPCR offers a sensitive, high-throughput approach to efficiently collect reliable data on the inoculum dynamics. A SYBR qPCR assay targeting the translation elongation factor 1α (EF1-α) gene of V. pirina was developed. The assay was validated based on specificity and sensitivity. The specificity was validated against 43 V. pirina isolates and 13 non-target fungal species. A qPCR offers a prospective tool which can be employed in various aspects of pathogenic studies.
Original languageEnglish
Title of host publicationProceedings of the 21st International Conference on Organic Fruit-Growing
PublisherFordergemeinschaft Okologischer Obstbau e.V. (FOKO)
Pages69-70
Number of pages2
Publication statusPublished - 2024
Event21st International Conference on Organic Fruit-Growing - Filderstadt, Germany
Duration: 19 Feb 202421 Feb 2024

Conference/symposium

Conference/symposium21st International Conference on Organic Fruit-Growing
Country/TerritoryGermany
CityFilderstadt
Period19/02/2421/02/24

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