Real-time imaging of microparticles and living cells with CMOS nanocapacitor arrays

C. Laborde, F. Pittino, H.A. Verhoeven, S.G. Lemay, L. Selmi, M.A. Jongsma, F.P. Widdershoven

Research output: Contribution to journalArticleAcademicpeer-review

48 Citations (Scopus)

Abstract

Massively parallel, label free biosensing platforms can in principle be realized by combining all-electrical detection with low-cost integrated circuits. Examples include field-effect transistor (FET) arrays used for mapping neuronal signals1,2 and DNA sequencing3,4. Despite these remarkable successes, however, bioelectronics has so far failed to deliver a broadly applicable biosensing platform, in no small part because DC or low-frequency signals do not allow probing beyond the electrical double layer (EDL) formed by screening salt ions5-8. This entails that, under physiological conditions, the sensing of target analytes located even a short distance from the sensor (~1 nm) is severely hampered. Here we demonstrate theoretically and experimentally the ability to detect and image microscale entities at long range under physiological salt conditions using high-frequency impedance spectroscopy with unprecedented spatial and temporal resolution. The assay employs a large-scale, high-density array of nanoelectrodes integrated with CMOS electronics on a single chip. The sensor response depends on the electrical properties of the analyte, allowing impedance-based fingerprinting. We also image the dynamic attachment and micromotion of BEAS, THP1 and MCF7 cancer cell lines in real time at submicron resolution in growth medium, demonstrating the potential of the platform for label/tracer-free high-throughput screening of anti-tumor drug candidates.
Original languageEnglish
Pages (from-to)791-795
JournalNature Nanotechnology
Volume10
DOIs
Publication statusPublished - 2015

Fingerprint

microparticles
Labels
CMOS
Screening
platforms
Salts
Cells
Imaging techniques
Sensors
screening
Field effect transistors
impedance
salts
Integrated circuits
Tumors
Assays
Electric properties
DNA
Electronic equipment
sensors

Cite this

Laborde, C. ; Pittino, F. ; Verhoeven, H.A. ; Lemay, S.G. ; Selmi, L. ; Jongsma, M.A. ; Widdershoven, F.P. / Real-time imaging of microparticles and living cells with CMOS nanocapacitor arrays. In: Nature Nanotechnology. 2015 ; Vol. 10. pp. 791-795.
@article{8ee12e2c35984433aec2f314f710ff2e,
title = "Real-time imaging of microparticles and living cells with CMOS nanocapacitor arrays",
abstract = "Massively parallel, label free biosensing platforms can in principle be realized by combining all-electrical detection with low-cost integrated circuits. Examples include field-effect transistor (FET) arrays used for mapping neuronal signals1,2 and DNA sequencing3,4. Despite these remarkable successes, however, bioelectronics has so far failed to deliver a broadly applicable biosensing platform, in no small part because DC or low-frequency signals do not allow probing beyond the electrical double layer (EDL) formed by screening salt ions5-8. This entails that, under physiological conditions, the sensing of target analytes located even a short distance from the sensor (~1 nm) is severely hampered. Here we demonstrate theoretically and experimentally the ability to detect and image microscale entities at long range under physiological salt conditions using high-frequency impedance spectroscopy with unprecedented spatial and temporal resolution. The assay employs a large-scale, high-density array of nanoelectrodes integrated with CMOS electronics on a single chip. The sensor response depends on the electrical properties of the analyte, allowing impedance-based fingerprinting. We also image the dynamic attachment and micromotion of BEAS, THP1 and MCF7 cancer cell lines in real time at submicron resolution in growth medium, demonstrating the potential of the platform for label/tracer-free high-throughput screening of anti-tumor drug candidates.",
author = "C. Laborde and F. Pittino and H.A. Verhoeven and S.G. Lemay and L. Selmi and M.A. Jongsma and F.P. Widdershoven",
year = "2015",
doi = "10.1038/nnano.2015.163",
language = "English",
volume = "10",
pages = "791--795",
journal = "Nature Nanotechnology",
issn = "1748-3387",
publisher = "Nature Publishing Group",

}

Real-time imaging of microparticles and living cells with CMOS nanocapacitor arrays. / Laborde, C.; Pittino, F.; Verhoeven, H.A.; Lemay, S.G.; Selmi, L.; Jongsma, M.A.; Widdershoven, F.P.

In: Nature Nanotechnology, Vol. 10, 2015, p. 791-795.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Real-time imaging of microparticles and living cells with CMOS nanocapacitor arrays

AU - Laborde, C.

AU - Pittino, F.

AU - Verhoeven, H.A.

AU - Lemay, S.G.

AU - Selmi, L.

AU - Jongsma, M.A.

AU - Widdershoven, F.P.

PY - 2015

Y1 - 2015

N2 - Massively parallel, label free biosensing platforms can in principle be realized by combining all-electrical detection with low-cost integrated circuits. Examples include field-effect transistor (FET) arrays used for mapping neuronal signals1,2 and DNA sequencing3,4. Despite these remarkable successes, however, bioelectronics has so far failed to deliver a broadly applicable biosensing platform, in no small part because DC or low-frequency signals do not allow probing beyond the electrical double layer (EDL) formed by screening salt ions5-8. This entails that, under physiological conditions, the sensing of target analytes located even a short distance from the sensor (~1 nm) is severely hampered. Here we demonstrate theoretically and experimentally the ability to detect and image microscale entities at long range under physiological salt conditions using high-frequency impedance spectroscopy with unprecedented spatial and temporal resolution. The assay employs a large-scale, high-density array of nanoelectrodes integrated with CMOS electronics on a single chip. The sensor response depends on the electrical properties of the analyte, allowing impedance-based fingerprinting. We also image the dynamic attachment and micromotion of BEAS, THP1 and MCF7 cancer cell lines in real time at submicron resolution in growth medium, demonstrating the potential of the platform for label/tracer-free high-throughput screening of anti-tumor drug candidates.

AB - Massively parallel, label free biosensing platforms can in principle be realized by combining all-electrical detection with low-cost integrated circuits. Examples include field-effect transistor (FET) arrays used for mapping neuronal signals1,2 and DNA sequencing3,4. Despite these remarkable successes, however, bioelectronics has so far failed to deliver a broadly applicable biosensing platform, in no small part because DC or low-frequency signals do not allow probing beyond the electrical double layer (EDL) formed by screening salt ions5-8. This entails that, under physiological conditions, the sensing of target analytes located even a short distance from the sensor (~1 nm) is severely hampered. Here we demonstrate theoretically and experimentally the ability to detect and image microscale entities at long range under physiological salt conditions using high-frequency impedance spectroscopy with unprecedented spatial and temporal resolution. The assay employs a large-scale, high-density array of nanoelectrodes integrated with CMOS electronics on a single chip. The sensor response depends on the electrical properties of the analyte, allowing impedance-based fingerprinting. We also image the dynamic attachment and micromotion of BEAS, THP1 and MCF7 cancer cell lines in real time at submicron resolution in growth medium, demonstrating the potential of the platform for label/tracer-free high-throughput screening of anti-tumor drug candidates.

U2 - 10.1038/nnano.2015.163

DO - 10.1038/nnano.2015.163

M3 - Article

VL - 10

SP - 791

EP - 795

JO - Nature Nanotechnology

JF - Nature Nanotechnology

SN - 1748-3387

ER -