Rapid Detection of Tetracyclines and Their 4-Epimer Derivatives from Poultry Meat with Bioluminescent Biosensor Bacteria

N.E. Virolainen, M.G. Pikkemaat, J.W.A. Elferink, M.T. Karp

    Research output: Contribution to journalArticleAcademicpeer-review

    57 Citations (Scopus)

    Abstract

    Tetracycline (TC) specific luminescent bacterial biosensors were used in a rapid TC residue assay sensitized to meet the EU maximum residue limit (MRL) for TC residues in poultry muscle tissue (100/mu g kg(-1)) by membrane-permeabilizing and chelating agents polymyxin B and EDTA. Sensitivities of 5 ng g(-1) for doxycycline, 7.5 ng g(-1) for chlortetracycline, and 25 ng g(-1) for tetracycline and oxytetracycline were reached. Except for doxycycline, the MRLs of these tetracyclines include their 4-epimer metabolites. In the biosensor assay, all four 4-epimers showed induction capacity and antimicrobial activity, and antimicrobial activity was also observed in the inhibition assay, although with lower efficiency than that of the corresponding parent compound in both assays. The biosensor assay is an inexpensive and rapid high-throughput screening method for the detection of 4-epimer TC residues along with their parent compounds.
    Original languageEnglish
    Pages (from-to)11065-11070
    JournalJournal of Agricultural and Food Chemistry
    Volume56
    Issue number23
    DOIs
    Publication statusPublished - 2008

    Keywords

    • tet repressor
    • sensor strain
    • residues
    • milk
    • performance
    • resistance
    • tests

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