Rapid antibody selection using surface plasmon resonance for high-speed and sensitive hazelnut lateral flow prototypes

Georgina M.S. Ross, Maria G.E.G. Bremer, Jan H. Wichers, Aart Van Amerongen, Michel W.F. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.

LanguageEnglish
Article number130
JournalBiosensors
Volume8
Issue number4
DOIs
Publication statusPublished - 14 Dec 2018

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Corylus
Surface Plasmon Resonance
Surface plasmon resonance
Immunoassay
Antibodies
Monoclonal Antibodies
Association reactions
Screening
Kinetics
Smartphones
Biomolecules
Application programs
Allergens
Labeling
Purification
Labels
Assays
Carbon
Nanoparticles
Ligands

Keywords

  • Antibody selection
  • Carbon nanoparticles
  • Food allergen
  • High-speed lateral flow immunoassay
  • Smartphone detection
  • Surface plasmon resonance

Cite this

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title = "Rapid antibody selection using surface plasmon resonance for high-speed and sensitive hazelnut lateral flow prototypes",
abstract = "Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.",
keywords = "Antibody selection, Carbon nanoparticles, Food allergen, High-speed lateral flow immunoassay, Smartphone detection, Surface plasmon resonance",
author = "Ross, {Georgina M.S.} and Bremer, {Maria G.E.G.} and Wichers, {Jan H.} and {Van Amerongen}, Aart and Nielen, {Michel W.F.}",
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Rapid antibody selection using surface plasmon resonance for high-speed and sensitive hazelnut lateral flow prototypes. / Ross, Georgina M.S.; Bremer, Maria G.E.G.; Wichers, Jan H.; Van Amerongen, Aart; Nielen, Michel W.F.

In: Biosensors, Vol. 8, No. 4, 130, 14.12.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Rapid antibody selection using surface plasmon resonance for high-speed and sensitive hazelnut lateral flow prototypes

AU - Ross, Georgina M.S.

AU - Bremer, Maria G.E.G.

AU - Wichers, Jan H.

AU - Van Amerongen, Aart

AU - Nielen, Michel W.F.

PY - 2018/12/14

Y1 - 2018/12/14

N2 - Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.

AB - Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.

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KW - Carbon nanoparticles

KW - Food allergen

KW - High-speed lateral flow immunoassay

KW - Smartphone detection

KW - Surface plasmon resonance

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