Rapid and sustained systemic circulation of conjugated gut microbiol catabolites after single-dose black tea extract consumption

J.P.M. van Duynhoven, J.J.J. van der Hooft, F.A. van Dorsten, S. Peters, M. Foltz, V. Gomez-Roldan, J.J.M. Vervoort, R.C.H. de Vos

Research output: Contribution to journalArticleAcademicpeer-review

34 Citations (Scopus)

Abstract

Gut microbial catabolites of black tea polyphenols (BTPs) have been proposed to exert beneficial cardiovascular bioactivity. This hypothesis is difficult to verify because the conjugation patterns and pharmacokinetics of these catabolites are largely unknown. The objective of our study was to identify, quantify, and assess the pharmacokinetics of conjugated BTP metabolites in plasma of healthy humans by means of an a priori untargeted LC–MS-based metabolomics approach. In a randomized, open, placebo-controlled, crossover study, 12 healthy men consumed a single bolus of black tea extract (BTE) or a placebo. The relative and, in several cases, absolute concentrations of a wide range of metabolites were determined using U(H)PLC-LTQ-Orbitrap-FTMS. Following BTE consumption, a kinetic response in plasma was observed for 59 BTP metabolites, 11 of these in a quantitative manner. Conjugated and unconjugated catechins appeared in plasma without delay, at 2–4 h, followed by a range of microbial catabolites. Interindividual variation in response was greater for gut microbial catabolites than for directly absorbed BTPs. The rapid and sustained circulation of conjugated catabolites suggests that these compounds may be particularly relevant to proposed health benefits of BTE. Their presence and effects may depend on individual variation in catabolic capacity of the gut microbiota.
Original languageEnglish
Pages (from-to)2668-2678
JournalJournal of Proteome Research
Volume13
Issue number5
DOIs
Publication statusPublished - 2014

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Tea
Polyphenols
Metabolites
Pharmacokinetics
Plasmas
Placebos
Metabolomics
Catechin
Insurance Benefits
Programmable logic controllers
Bioactivity
Cross-Over Studies
Health
Kinetics

Keywords

  • randomized controlled-trial
  • red wine/grape juice
  • green tea
  • mass-spectrometry
  • in-vitro
  • dietary polyphenols
  • blood-pressure
  • catechins
  • metabolites
  • humans

Cite this

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title = "Rapid and sustained systemic circulation of conjugated gut microbiol catabolites after single-dose black tea extract consumption",
abstract = "Gut microbial catabolites of black tea polyphenols (BTPs) have been proposed to exert beneficial cardiovascular bioactivity. This hypothesis is difficult to verify because the conjugation patterns and pharmacokinetics of these catabolites are largely unknown. The objective of our study was to identify, quantify, and assess the pharmacokinetics of conjugated BTP metabolites in plasma of healthy humans by means of an a priori untargeted LC–MS-based metabolomics approach. In a randomized, open, placebo-controlled, crossover study, 12 healthy men consumed a single bolus of black tea extract (BTE) or a placebo. The relative and, in several cases, absolute concentrations of a wide range of metabolites were determined using U(H)PLC-LTQ-Orbitrap-FTMS. Following BTE consumption, a kinetic response in plasma was observed for 59 BTP metabolites, 11 of these in a quantitative manner. Conjugated and unconjugated catechins appeared in plasma without delay, at 2–4 h, followed by a range of microbial catabolites. Interindividual variation in response was greater for gut microbial catabolites than for directly absorbed BTPs. The rapid and sustained circulation of conjugated catabolites suggests that these compounds may be particularly relevant to proposed health benefits of BTE. Their presence and effects may depend on individual variation in catabolic capacity of the gut microbiota.",
keywords = "randomized controlled-trial, red wine/grape juice, green tea, mass-spectrometry, in-vitro, dietary polyphenols, blood-pressure, catechins, metabolites, humans",
author = "{van Duynhoven}, J.P.M. and {van der Hooft}, J.J.J. and {van Dorsten}, F.A. and S. Peters and M. Foltz and V. Gomez-Roldan and J.J.M. Vervoort and {de Vos}, R.C.H.",
year = "2014",
doi = "10.1021/pr5001253",
language = "English",
volume = "13",
pages = "2668--2678",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "5",

}

Rapid and sustained systemic circulation of conjugated gut microbiol catabolites after single-dose black tea extract consumption. / van Duynhoven, J.P.M.; van der Hooft, J.J.J.; van Dorsten, F.A.; Peters, S.; Foltz, M.; Gomez-Roldan, V.; Vervoort, J.J.M.; de Vos, R.C.H.

In: Journal of Proteome Research, Vol. 13, No. 5, 2014, p. 2668-2678.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Rapid and sustained systemic circulation of conjugated gut microbiol catabolites after single-dose black tea extract consumption

AU - van Duynhoven, J.P.M.

AU - van der Hooft, J.J.J.

AU - van Dorsten, F.A.

AU - Peters, S.

AU - Foltz, M.

AU - Gomez-Roldan, V.

AU - Vervoort, J.J.M.

AU - de Vos, R.C.H.

PY - 2014

Y1 - 2014

N2 - Gut microbial catabolites of black tea polyphenols (BTPs) have been proposed to exert beneficial cardiovascular bioactivity. This hypothesis is difficult to verify because the conjugation patterns and pharmacokinetics of these catabolites are largely unknown. The objective of our study was to identify, quantify, and assess the pharmacokinetics of conjugated BTP metabolites in plasma of healthy humans by means of an a priori untargeted LC–MS-based metabolomics approach. In a randomized, open, placebo-controlled, crossover study, 12 healthy men consumed a single bolus of black tea extract (BTE) or a placebo. The relative and, in several cases, absolute concentrations of a wide range of metabolites were determined using U(H)PLC-LTQ-Orbitrap-FTMS. Following BTE consumption, a kinetic response in plasma was observed for 59 BTP metabolites, 11 of these in a quantitative manner. Conjugated and unconjugated catechins appeared in plasma without delay, at 2–4 h, followed by a range of microbial catabolites. Interindividual variation in response was greater for gut microbial catabolites than for directly absorbed BTPs. The rapid and sustained circulation of conjugated catabolites suggests that these compounds may be particularly relevant to proposed health benefits of BTE. Their presence and effects may depend on individual variation in catabolic capacity of the gut microbiota.

AB - Gut microbial catabolites of black tea polyphenols (BTPs) have been proposed to exert beneficial cardiovascular bioactivity. This hypothesis is difficult to verify because the conjugation patterns and pharmacokinetics of these catabolites are largely unknown. The objective of our study was to identify, quantify, and assess the pharmacokinetics of conjugated BTP metabolites in plasma of healthy humans by means of an a priori untargeted LC–MS-based metabolomics approach. In a randomized, open, placebo-controlled, crossover study, 12 healthy men consumed a single bolus of black tea extract (BTE) or a placebo. The relative and, in several cases, absolute concentrations of a wide range of metabolites were determined using U(H)PLC-LTQ-Orbitrap-FTMS. Following BTE consumption, a kinetic response in plasma was observed for 59 BTP metabolites, 11 of these in a quantitative manner. Conjugated and unconjugated catechins appeared in plasma without delay, at 2–4 h, followed by a range of microbial catabolites. Interindividual variation in response was greater for gut microbial catabolites than for directly absorbed BTPs. The rapid and sustained circulation of conjugated catabolites suggests that these compounds may be particularly relevant to proposed health benefits of BTE. Their presence and effects may depend on individual variation in catabolic capacity of the gut microbiota.

KW - randomized controlled-trial

KW - red wine/grape juice

KW - green tea

KW - mass-spectrometry

KW - in-vitro

KW - dietary polyphenols

KW - blood-pressure

KW - catechins

KW - metabolites

KW - humans

U2 - 10.1021/pr5001253

DO - 10.1021/pr5001253

M3 - Article

VL - 13

SP - 2668

EP - 2678

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 5

ER -