Rapid and efficient purification method for small, hydrophobic, cationicbacteriocins: purification of lactococcin B and pediocin PA-1.

K. Venema, M.L. Chikindas, J.F.M.L. Seegers, A.J. Haandrikman, G. Venema, J. Kok

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)

Abstract

The bacteriocins lactococcin B and pediocin PA-1 were purified by ethanol precipitation, preparative isoelectric focusing, and ultrafiltration. The procedure reproducibly leads to high final yields in comparison to the generally low yields obtained by column chromatography. Specifically, during isoelectric focusing no loss of activity occurs. The method, in general, should be applicable to small, hydrophobic, cationic bacteriocins.
Original languageEnglish
Pages (from-to)305-309
JournalApplied and Environmental Microbiology
Volume63
Publication statusPublished - 1997

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