Agrobacterium tumefaciens-mediated transformation (ATMT) has become an important tool for functional genomics in fungi. ATMT-based approaches such as random insertional mutagenesis and targeted knockout are widely used for gene functional analysis in plant-pathogen interactions. Here, we describe a protocol for the identification of pathogenicity and virulence genes through random insertional mutagenesis using the fungal wilt pathogen Verticillium dahliae as an example for the protocol.
|Title of host publication||Plant Fungal Pathogens: Methods and Protocols|
|Editors||M.D. Bolton, B.P.H.J. Thomma|
|Place of Publication||New York, USA|
|Number of pages||648|
|Publication status||Published - 2012|
|Name||Methods in Molecular Biology|
|Publisher||Humana Press, c/o Springer Science+Business Media|