Random insertional mutagenesis in fungal genomes to identify virulence factors

P. Santhanam

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

11 Citations (Scopus)


Agrobacterium tumefaciens-mediated transformation (ATMT) has become an important tool for functional genomics in fungi. ATMT-based approaches such as random insertional mutagenesis and targeted knockout are widely used for gene functional analysis in plant-pathogen interactions. Here, we describe a protocol for the identification of pathogenicity and virulence genes through random insertional mutagenesis using the fungal wilt pathogen Verticillium dahliae as an example for the protocol.
Original languageEnglish
Title of host publicationPlant Fungal Pathogens: Methods and Protocols
EditorsM.D. Bolton, B.P.H.J. Thomma
Place of PublicationNew York, USA
Number of pages648
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
PublisherHumana Press, c/o Springer Science+Business Media


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