Race specific resistance againt Phytophthora infestans in potato is controlled by more genetic factors than only R-genes.

A. El-Kharbotly, A. Pereira, W.J. Stiekema, E. Jacobsen

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

For RFLP mapping of R-genes, determining resistance to specific races of Phytophthora infestans in tetraploid potato, it is necessary to develop well segregating populations at the 2x level. During mapping studies, evidence was obtained that more genetic factor(s) are involved in the expression of R-genes than conventionally believed. Two experiments are described in which such an additional genetic factor was suppressing or enhancing the expression of unknown R nand R ifactors. R nand R iappeared to be present in the investigated plant material, containing R4 and R10, or in one of the susceptible crossing parents. In a third experiment, the expression and the segregation of the well known R1 gene was influenced by an additional genetic factor. In that case there were indications for a dominant suppressor. This was established by the selection of susceptible plants carrying a RFLP allele of probe GP21 closely linked to R1. In three of the four F1 populations, resulting from crosses between such susceptible plants and susceptible tester plants, resistnat progenies were found. The resistance appeared to be R1-specific. This clearly indicates that in three of the four investigated susceptible plants, the R1 gene was still present but not expressed.
Original languageEnglish
Pages (from-to)331-336
JournalEuphytica
Volume90
DOIs
Publication statusPublished - 1996

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Phytophthora infestans
vpr Genes
vertical resistance
Solanum tuberosum
potatoes
Restriction Fragment Length Polymorphisms
genes
restriction fragment length polymorphism
Plant Genes
Tetraploidy
gene segregation
Population
Parents
Alleles
tetraploidy
alleles
Genes

Cite this

@article{5e91dc7de4684c48923ade689df858d9,
title = "Race specific resistance againt Phytophthora infestans in potato is controlled by more genetic factors than only R-genes.",
abstract = "For RFLP mapping of R-genes, determining resistance to specific races of Phytophthora infestans in tetraploid potato, it is necessary to develop well segregating populations at the 2x level. During mapping studies, evidence was obtained that more genetic factor(s) are involved in the expression of R-genes than conventionally believed. Two experiments are described in which such an additional genetic factor was suppressing or enhancing the expression of unknown R nand R ifactors. R nand R iappeared to be present in the investigated plant material, containing R4 and R10, or in one of the susceptible crossing parents. In a third experiment, the expression and the segregation of the well known R1 gene was influenced by an additional genetic factor. In that case there were indications for a dominant suppressor. This was established by the selection of susceptible plants carrying a RFLP allele of probe GP21 closely linked to R1. In three of the four F1 populations, resulting from crosses between such susceptible plants and susceptible tester plants, resistnat progenies were found. The resistance appeared to be R1-specific. This clearly indicates that in three of the four investigated susceptible plants, the R1 gene was still present but not expressed.",
author = "A. El-Kharbotly and A. Pereira and W.J. Stiekema and E. Jacobsen",
year = "1996",
doi = "10.1007/BF00027484",
language = "English",
volume = "90",
pages = "331--336",
journal = "Euphytica",
issn = "0014-2336",
publisher = "Springer Verlag",

}

Race specific resistance againt Phytophthora infestans in potato is controlled by more genetic factors than only R-genes. / El-Kharbotly, A.; Pereira, A.; Stiekema, W.J.; Jacobsen, E.

In: Euphytica, Vol. 90, 1996, p. 331-336.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Race specific resistance againt Phytophthora infestans in potato is controlled by more genetic factors than only R-genes.

AU - El-Kharbotly, A.

AU - Pereira, A.

AU - Stiekema, W.J.

AU - Jacobsen, E.

PY - 1996

Y1 - 1996

N2 - For RFLP mapping of R-genes, determining resistance to specific races of Phytophthora infestans in tetraploid potato, it is necessary to develop well segregating populations at the 2x level. During mapping studies, evidence was obtained that more genetic factor(s) are involved in the expression of R-genes than conventionally believed. Two experiments are described in which such an additional genetic factor was suppressing or enhancing the expression of unknown R nand R ifactors. R nand R iappeared to be present in the investigated plant material, containing R4 and R10, or in one of the susceptible crossing parents. In a third experiment, the expression and the segregation of the well known R1 gene was influenced by an additional genetic factor. In that case there were indications for a dominant suppressor. This was established by the selection of susceptible plants carrying a RFLP allele of probe GP21 closely linked to R1. In three of the four F1 populations, resulting from crosses between such susceptible plants and susceptible tester plants, resistnat progenies were found. The resistance appeared to be R1-specific. This clearly indicates that in three of the four investigated susceptible plants, the R1 gene was still present but not expressed.

AB - For RFLP mapping of R-genes, determining resistance to specific races of Phytophthora infestans in tetraploid potato, it is necessary to develop well segregating populations at the 2x level. During mapping studies, evidence was obtained that more genetic factor(s) are involved in the expression of R-genes than conventionally believed. Two experiments are described in which such an additional genetic factor was suppressing or enhancing the expression of unknown R nand R ifactors. R nand R iappeared to be present in the investigated plant material, containing R4 and R10, or in one of the susceptible crossing parents. In a third experiment, the expression and the segregation of the well known R1 gene was influenced by an additional genetic factor. In that case there were indications for a dominant suppressor. This was established by the selection of susceptible plants carrying a RFLP allele of probe GP21 closely linked to R1. In three of the four F1 populations, resulting from crosses between such susceptible plants and susceptible tester plants, resistnat progenies were found. The resistance appeared to be R1-specific. This clearly indicates that in three of the four investigated susceptible plants, the R1 gene was still present but not expressed.

U2 - 10.1007/BF00027484

DO - 10.1007/BF00027484

M3 - Article

VL - 90

SP - 331

EP - 336

JO - Euphytica

JF - Euphytica

SN - 0014-2336

ER -