Abstract
Given the positive results of quercetin in in vitro genotoxicity studies, the in vivo genotoxic properties of this important dietary flavonoid warrant testing, especially considering possible high intake via widely available food supplements. Here, this was done by transcriptome analyses of the most relevant tissues, liver and small intestine, of quercetin supplemented mice. Quercetin (0.33%) supplemented to a high-fat diet was administered to mice during 12 weeks. Serum alanine aminotransferase and aspartate aminotransferase levels revealed no indications for hepatotoxicity. Microarray pathway analysis of liver and small intestine showed no regulation of genotoxicity related pathways. Analysis of DNA damage related genes also did not point at genotoxicity. Furthermore, a published classifier set of transcripts for identifying genotoxic compounds did not indicate genotoxicity. Only two transcripts of the classifier set were regulated, but in the opposite direction compared with the genotoxic compounds 2-acetylaminofluorene (2-AAF) and aflatoxin B1 (AFB1). Based on the weight of evidence of three different types of analysis, we conclude that supplementation with quercetin at ~350¿mg/kg bw/day for 12 weeks in mice showed no up-regulation of genotoxicity related pathways in liver and small intestine.
Original language | English |
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Pages (from-to) | 34-39 |
Journal | Food and Chemical Toxicology |
Volume | 81 |
DOIs | |
Publication status | Published - 2015 |
Keywords
- in-vivo
- reverse mutation
- bone-marrow
- dna-damage
- flavonoids
- rats
- cells
- carcinogenicity
- mutagenicity
- polyphenols
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Quercetin tests negative for genotoxicity in transcriptome analyses of liver and small intestine of mice
van den Hil, E. F. (Creator), van Schothorst, E. M. (Creator), van der Stelt, I. (Creator), Keijer, J. (Creator) & Rietjens, I. M. C. M. (Creator), Wageningen UR, 6 Aug 2015
http://ctdbase.org/detail.go?type=reference&acc=25862958
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Quercetin showed no genotoxicity by transcriptomic analysis in liver and small intestine of mice
Hoek, E. (Creator), van Schothorst, E. (Creator) & Keijer, J. (Creator), Wageningen University, 1 Oct 2015
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE63227
Dataset