Quantitative trait locus analysis of nitrogen use efficiency in barley (Hordeum vulgare L.)

G.A. Kindu, J. Tang, X. Yin, P.C. Struik

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Quantitative trait locus (QTL) analysis of nitrogen use efficiency (NUE) of barley (Hordeum vulgare L.) was conducted on data generated from two pot experiments carried out in 2005 (using four nitrogen rates) and 2008 (with three rates) with AFLP markers and 94 recombinant inbred lines (RILs) of the Prisma 9 Apex mapping population. In total 41 QTLs were detected on 6 chromosomes and for 18 traits in both trials. About 95 % of the detected QTLs were with major additive effects. The percentage of variance accounted for by individual QTLs in the multiple QTL mapping model ranged from 8.4 to 54.4 %across all mapped traits in both years. Fifteen QTLs were related to NUE and its components; most of these QTLs were detected at lower nitrogen rates and none at the highest rate in both trials. These QTLs were found on Chromosomes 3(3H) and 7(5H) in 2005 and Chromosome 2(2H) in 2008. Except for the QTLs of plant height and NUE based on grain yield, none of the QTLs which were detected for a given trait in 2005, expressed themselves in 2008 irrespective of the nitrogen levels. QTLs controlling some traits were colocated in each year, and QTLs for many traits were detected on the same chromosome and close to the denso locus. Further research is needed to investigate the possibility to reduce nitrogen fertilizer requirements through breeding while maintaining high yield of barley.
Original languageEnglish
Pages (from-to)207-221
JournalEuphytica
Volume199
Issue number1-2
DOIs
Publication statusPublished - 2014

Keywords

  • recombinant inbred lines
  • controlling flowering time
  • triticum-aestivum l.
  • spring barley
  • qtl analysis
  • experimental populations
  • genetic architecture
  • crop production
  • linkage maps
  • major genes

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