Quantitative trait loci for glucosinolate accumulation in Brassica rapa leaves

P. Lou, Jianjun Jianjun Zhao, Hongju He, C.J. Hanhart, D. Pino del Carpio, R. Verkerk, J.B.M. Custers, M. Koornneef, A.B. Bonnema

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53 Citations (Scopus)

Abstract

Glucosinolates and their breakdown products have been recognized for their effects on plant defense, human health, flavor and taste of cruciferous vegetables. Despite this importance, little is known about the regulation of the biosynthesis and degradation in Brassica rapa. Here, the identification of quantitative trait loci (QTL) for glucosinolate accumulation in B. rapa leaves in two novel segregating double haploid (DH) populations is reported: DH38, derived from a cross between yellow sarson R500 and pak choi variety HK Naibaicai; and DH30, from a cross between yellow sarson R500 and Kairyou Hakata, a Japanese vegetable turnip variety. An integrated map of 1068 cM with 10 linkage groups, assigned to the international agreed nomenclature, is developed based on the two individual DH maps with the common parent using amplified fragment length polymorphism (AFLP) and single sequence repeat (SSR) markers. Eight different glucosinolate compounds were detected in parents and F1s of the DH populations and found to segregate quantitatively in the DH populations. QTL analysis identified 16 loci controlling aliphatic glucosinolate accumulation, three loci controlling total indolic glucosinolate concentration and three loci regulating aromatic glucosinolate concentrations. Both comparative genomic analyses based on Arabidopsis¿Brassica rapa synteny and mapping of candidate orthologous genes in B. rapa allowed the selection of genes involved in the glucosinolate biosynthesis pathway that may account for the identified QTL
Original languageEnglish
Pages (from-to)1017-1032
JournalNew Phytologist
Volume179
Issue number4
DOIs
Publication statusPublished - 2008

Keywords

  • genetic-linkage map
  • arabidopsis-thaliana
  • auxin homeostasis
  • comparative genomics
  • insect resistance
  • natural variation
  • flowering time
  • biosynthesis
  • sequence
  • napus

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