Quantitative trait local analysis of growth-related traits in a new Arabidopsis recombinant inbred population

M.E.M. El-Lithy, E.J.M. Clerkx, G.J. Ruijs, M. Koornneef, D. Vreugdenhil

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Abstract

Arabidopsis natural variation was used to analyze the genetics of plant growth rate. Screening of 22 accessions revealed a large variation for seed weight, plant dry weight and relative growth rate but not for water content. A positive correlation was observed between seed weight and plant area 10 d after planting, suggesting that seed weight affects plant growth during early phases of development. During later stages of plant growth this correlation was not significant, indicating that other factors determine growth rate during this phase. Quantitative trait locus (QTL) analysis, using 114 (F9 generation) recombinant inbred lines derived from the cross between Landsberg erecta (Ler, from Poland) and Shakdara (Sha, from Tadjikistan), revealed QTLs for seed weight, plant area, dry weight, relative growth rate, chlorophyll fluorescence, flowering time, and flowering-related traits. Growth traits (plant area, dry weight, and relative growth rate) colocated at five genomic regions. At the bottom of chromosome 5, colocation was found of QTLs for leaf area, leaf initiation speed, specific leaf area, and chlorophyll fluorescence but not for dry weight, indicating that this locus might be involved in leaf development. No consistent relation between growth traits and flowering time was observed despite some colocations. Some of the QTLs detected for flowering time overlapped with loci detected in other recombinant inbred line populations, but also new loci were identified. This study shows that Arabidopsis can successfully be used to study the genetic basis of complex traits like plant growth rate.
LanguageEnglish
Pages444-458
JournalPlant Physiology
Volume135
Issue number1
DOIs
Publication statusPublished - 2004

Fingerprint

quantitative traits
Arabidopsis
Weights and Measures
Growth
Population
quantitative trait loci
Seeds
plant growth
flowering
growth traits
Chlorophyll
inbred lines
loci
leaf area
seeds
fluorescence
Tajikistan
planting seed
Fluorescence
chlorophyll

Keywords

  • natural allelic variation
  • late-flowering mutants
  • plant-growth
  • image-analysis
  • landsberg erecta
  • thaliana
  • time
  • accessions
  • gene
  • qtl

Cite this

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title = "Quantitative trait local analysis of growth-related traits in a new Arabidopsis recombinant inbred population",
abstract = "Arabidopsis natural variation was used to analyze the genetics of plant growth rate. Screening of 22 accessions revealed a large variation for seed weight, plant dry weight and relative growth rate but not for water content. A positive correlation was observed between seed weight and plant area 10 d after planting, suggesting that seed weight affects plant growth during early phases of development. During later stages of plant growth this correlation was not significant, indicating that other factors determine growth rate during this phase. Quantitative trait locus (QTL) analysis, using 114 (F9 generation) recombinant inbred lines derived from the cross between Landsberg erecta (Ler, from Poland) and Shakdara (Sha, from Tadjikistan), revealed QTLs for seed weight, plant area, dry weight, relative growth rate, chlorophyll fluorescence, flowering time, and flowering-related traits. Growth traits (plant area, dry weight, and relative growth rate) colocated at five genomic regions. At the bottom of chromosome 5, colocation was found of QTLs for leaf area, leaf initiation speed, specific leaf area, and chlorophyll fluorescence but not for dry weight, indicating that this locus might be involved in leaf development. No consistent relation between growth traits and flowering time was observed despite some colocations. Some of the QTLs detected for flowering time overlapped with loci detected in other recombinant inbred line populations, but also new loci were identified. This study shows that Arabidopsis can successfully be used to study the genetic basis of complex traits like plant growth rate.",
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author = "M.E.M. El-Lithy and E.J.M. Clerkx and G.J. Ruijs and M. Koornneef and D. Vreugdenhil",
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language = "English",
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pages = "444--458",
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Quantitative trait local analysis of growth-related traits in a new Arabidopsis recombinant inbred population. / El-Lithy, M.E.M.; Clerkx, E.J.M.; Ruijs, G.J.; Koornneef, M.; Vreugdenhil, D.

In: Plant Physiology, Vol. 135, No. 1, 2004, p. 444-458.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Quantitative trait local analysis of growth-related traits in a new Arabidopsis recombinant inbred population

AU - El-Lithy, M.E.M.

AU - Clerkx, E.J.M.

AU - Ruijs, G.J.

AU - Koornneef, M.

AU - Vreugdenhil, D.

PY - 2004

Y1 - 2004

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AB - Arabidopsis natural variation was used to analyze the genetics of plant growth rate. Screening of 22 accessions revealed a large variation for seed weight, plant dry weight and relative growth rate but not for water content. A positive correlation was observed between seed weight and plant area 10 d after planting, suggesting that seed weight affects plant growth during early phases of development. During later stages of plant growth this correlation was not significant, indicating that other factors determine growth rate during this phase. Quantitative trait locus (QTL) analysis, using 114 (F9 generation) recombinant inbred lines derived from the cross between Landsberg erecta (Ler, from Poland) and Shakdara (Sha, from Tadjikistan), revealed QTLs for seed weight, plant area, dry weight, relative growth rate, chlorophyll fluorescence, flowering time, and flowering-related traits. Growth traits (plant area, dry weight, and relative growth rate) colocated at five genomic regions. At the bottom of chromosome 5, colocation was found of QTLs for leaf area, leaf initiation speed, specific leaf area, and chlorophyll fluorescence but not for dry weight, indicating that this locus might be involved in leaf development. No consistent relation between growth traits and flowering time was observed despite some colocations. Some of the QTLs detected for flowering time overlapped with loci detected in other recombinant inbred line populations, but also new loci were identified. This study shows that Arabidopsis can successfully be used to study the genetic basis of complex traits like plant growth rate.

KW - natural allelic variation

KW - late-flowering mutants

KW - plant-growth

KW - image-analysis

KW - landsberg erecta

KW - thaliana

KW - time

KW - accessions

KW - gene

KW - qtl

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T2 - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 1

ER -