Quantitative resistance against Bemisia tabaci in Solanum pennellii: Genetics and metabolomics

F.H.W. van den Elsen, A.F. Lucatti, A.W. van Heusden, C. Broekgaarden, R. Mumm, M. Dicke, B. Vosman

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

The whitefly Bemisia tabaci is a serious threat in tomato cultivation worldwide as all varieties grown today are highly susceptible to this devastating herbivorous insect. Many accessions of the tomato wild relative Solanum pennellii show a high resistance towards B. tabaci. A mapping approach was used to elucidate the genetic background of whitefly-resistance related traits and associated biochemical traits in this species. Minor quantitative trait loci (QTLs) for whitefly adult survival (AS) and oviposition rate (OR) were identified and some were confirmed in an F2BC1 population, where they showed increased percentages of explained variance (more than 30%). Bulked segregant analyses on pools of whitefly-resistant and -susceptible F2 plants enabled the identification of metabolites that correlate either with resistance or susceptibility. Genetic mapping of these metabolites showed that a large number of them co-localize with whitefly-resistance QTLs. Some of these whitefly-resistance QTLs are hotspots for metabolite QTLs. Although a large number of metabolite QTLs correlated to whitefly resistance or susceptibility, most of them are yet unknown compounds and further studies are needed to identify the metabolic pathways and genes involved. The results indicate a direct genetic correlation between biochemical-based resistance characteristics and reduced whitefly incidence in S. pennellii.
Original languageEnglish
Pages (from-to)397-412
JournalJournal of Integrative Plant Biology
Volume58
Issue number4
DOIs
Publication statusPublished - 2016

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Solanum pennellii
Solanum
Hemiptera
Metabolomics
Bemisia tabaci
metabolomics
Metabolites
Aleyrodidae
Quantitative Trait Loci
quantitative trait loci
metabolites
Lycopersicon esculentum
Genes
tomatoes
Oviposition
Genetics
phytophagous insects
wild relatives
Metabolic Networks and Pathways
genetic background

Cite this

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title = "Quantitative resistance against Bemisia tabaci in Solanum pennellii: Genetics and metabolomics",
abstract = "The whitefly Bemisia tabaci is a serious threat in tomato cultivation worldwide as all varieties grown today are highly susceptible to this devastating herbivorous insect. Many accessions of the tomato wild relative Solanum pennellii show a high resistance towards B. tabaci. A mapping approach was used to elucidate the genetic background of whitefly-resistance related traits and associated biochemical traits in this species. Minor quantitative trait loci (QTLs) for whitefly adult survival (AS) and oviposition rate (OR) were identified and some were confirmed in an F2BC1 population, where they showed increased percentages of explained variance (more than 30{\%}). Bulked segregant analyses on pools of whitefly-resistant and -susceptible F2 plants enabled the identification of metabolites that correlate either with resistance or susceptibility. Genetic mapping of these metabolites showed that a large number of them co-localize with whitefly-resistance QTLs. Some of these whitefly-resistance QTLs are hotspots for metabolite QTLs. Although a large number of metabolite QTLs correlated to whitefly resistance or susceptibility, most of them are yet unknown compounds and further studies are needed to identify the metabolic pathways and genes involved. The results indicate a direct genetic correlation between biochemical-based resistance characteristics and reduced whitefly incidence in S. pennellii.",
author = "{van den Elsen}, F.H.W. and A.F. Lucatti and {van Heusden}, A.W. and C. Broekgaarden and R. Mumm and M. Dicke and B. Vosman",
year = "2016",
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journal = "Journal of Integrative Plant Biology",
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Quantitative resistance against Bemisia tabaci in Solanum pennellii: Genetics and metabolomics. / van den Elsen, F.H.W.; Lucatti, A.F.; van Heusden, A.W.; Broekgaarden, C.; Mumm, R.; Dicke, M.; Vosman, B.

In: Journal of Integrative Plant Biology, Vol. 58, No. 4, 2016, p. 397-412.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Quantitative resistance against Bemisia tabaci in Solanum pennellii: Genetics and metabolomics

AU - van den Elsen, F.H.W.

AU - Lucatti, A.F.

AU - van Heusden, A.W.

AU - Broekgaarden, C.

AU - Mumm, R.

AU - Dicke, M.

AU - Vosman, B.

PY - 2016

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N2 - The whitefly Bemisia tabaci is a serious threat in tomato cultivation worldwide as all varieties grown today are highly susceptible to this devastating herbivorous insect. Many accessions of the tomato wild relative Solanum pennellii show a high resistance towards B. tabaci. A mapping approach was used to elucidate the genetic background of whitefly-resistance related traits and associated biochemical traits in this species. Minor quantitative trait loci (QTLs) for whitefly adult survival (AS) and oviposition rate (OR) were identified and some were confirmed in an F2BC1 population, where they showed increased percentages of explained variance (more than 30%). Bulked segregant analyses on pools of whitefly-resistant and -susceptible F2 plants enabled the identification of metabolites that correlate either with resistance or susceptibility. Genetic mapping of these metabolites showed that a large number of them co-localize with whitefly-resistance QTLs. Some of these whitefly-resistance QTLs are hotspots for metabolite QTLs. Although a large number of metabolite QTLs correlated to whitefly resistance or susceptibility, most of them are yet unknown compounds and further studies are needed to identify the metabolic pathways and genes involved. The results indicate a direct genetic correlation between biochemical-based resistance characteristics and reduced whitefly incidence in S. pennellii.

AB - The whitefly Bemisia tabaci is a serious threat in tomato cultivation worldwide as all varieties grown today are highly susceptible to this devastating herbivorous insect. Many accessions of the tomato wild relative Solanum pennellii show a high resistance towards B. tabaci. A mapping approach was used to elucidate the genetic background of whitefly-resistance related traits and associated biochemical traits in this species. Minor quantitative trait loci (QTLs) for whitefly adult survival (AS) and oviposition rate (OR) were identified and some were confirmed in an F2BC1 population, where they showed increased percentages of explained variance (more than 30%). Bulked segregant analyses on pools of whitefly-resistant and -susceptible F2 plants enabled the identification of metabolites that correlate either with resistance or susceptibility. Genetic mapping of these metabolites showed that a large number of them co-localize with whitefly-resistance QTLs. Some of these whitefly-resistance QTLs are hotspots for metabolite QTLs. Although a large number of metabolite QTLs correlated to whitefly resistance or susceptibility, most of them are yet unknown compounds and further studies are needed to identify the metabolic pathways and genes involved. The results indicate a direct genetic correlation between biochemical-based resistance characteristics and reduced whitefly incidence in S. pennellii.

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