Quantitative Profiling of Oxylipins through Comprehensive LC-MS/MS Analysis: Application in cardiac surgery

K. Strassburg, A.M.L. Huijbrechts, K. Kortekaas, J. Lindeman, T.L. Pedersen, J.W. Newman, A. Dane, R. Berger, A. Brenkman, T. Hankemeier, J.P.M. van Duynhoven, E. Kalkhoven, R. Vreeken

Research output: Contribution to journalArticleAcademicpeer-review

151 Citations (Scopus)

Abstract

Oxylipins, including eicosanoids, affect a broad range of biological processes, such as the initiation and resolution of inflammation. These compounds, also referred to as lipid mediators, are (non-) enzymatically generated by oxidation of polyunsaturated fatty acids such as arachidonic acid (AA). A plethora of lipid mediators exist which makes the development of generic analytical methods challenging. Here we developed a robust and sensitive targeted analysis platform for oxylipins and applied it in a biological setting, using high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) operated in dynamic multiple reaction monitoring (dMRM). Besides the well-described AA metabolites, oxylipins derived from linoleic acid, dihomo-¿-linolenic acid, a-linolenic acid, eicosapentaenoic acid and docosahexaenoic acid were included. Our comprehensive platform allows the quantitative evaluation of approximately 100 oxylipins down to low nanomolar levels. Applicability of the analytical platform was demonstrated by analyzing plasma samples of patients undergoing cardiac surgery. Altered levels of some of the oxylipins, especially in certain monohydroxy fatty acids such as 12-HETE and 12-HEPE, were observed in samples collected before and 24 h after cardiac surgery. These findings indicate that this generic oxylipin profiling platform can be applied broadly to study these highly bioactive compounds in relation to human disease.
Original languageEnglish
Pages (from-to)1413-1426
Number of pages14
JournalAnalytical and Bioanalytical Chemistry
Volume404
Issue number5
DOIs
Publication statusPublished - 2012

Keywords

  • tandem mass-spectrometry
  • soluble epoxide hydrolase
  • arachidonic-acid
  • human plasma
  • lipidomic analysis
  • fatty-acids
  • in-vivo
  • dihydroxyeicosatrienoic acids
  • hydroxyeicosatetraenoic acids
  • simultaneous quantification

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