Quantitative ELISA for the detection of potato cyst nematodes in soil samples

A. Schots, F.J. Gommers, E. Egberts

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Monoclonal antibodies (McAbs) * are typifïed by uniform physical characteristics. The behaviour of McAbs in an immunoassay can therefore be predicted once the major binding parameters have been determined. Here, a method is described to characterize an indirect ELISA for the qualitative and quantitative determination of the potato cyst nematodes Globodera roslOchiensis and G. pallida. This assay makes use of McAbs with properly determined binding constants (K's). The practical implications of such a characterization are demonstrated, with special reference to the phenomenon of " cross-reactivity ". Experimental validation was performed using a routine to fit sigmoidal curves. The conditions to be fullfïlled for the correct application of the method are discussed.
Original languageEnglish
Pages (from-to)55-61
JournalFundamental and applied nematology
Volume15
Publication statusPublished - 1992

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