After tracking the kinematics of a moving mosquito from video, one needs to convert the tracking output into the kinematics data needed to answer the research question. Here, we provide general guidelines for how to do this by discussing how to quantify body position and orientation in the world reference frame and wing and leg orientation in the mosquito body reference frame. These guidelines should be adapted based on the goal of your research. To answer your research question, the resulting kinematics data must then be further analyzed. Because the nature of this analysis depends strongly on your specific research question, we refer to literature for designing these postprocessing routines.