Quantification of interferon signaling in avian cells

Joeri Kint*, Maria Forlenza

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Activation of the type I interferon (IFN) response is an essential defense mechanism against invading pathogens such as viruses. This chapter describes two protocols to quantify activation of the chicken IFN response through analysis of gene expression by real-time quantitative PCR and by quantification of bioactive IFN protein using a bioassay.

Original languageEnglish
Title of host publicationCoronaviruses: Methods and Protocols
PublisherSpringer New York LLC
Pages251-259
ISBN (Print)9781493924387, 9781493924370
DOIs
Publication statusPublished - 2015

Keywords

  • Bioassay
  • Chicken
  • Interferon
  • Real-time quantitative PCR

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    Kint, J., & Forlenza, M. (2015). Quantification of interferon signaling in avian cells. In Coronaviruses: Methods and Protocols (pp. 251-259). Springer New York LLC. https://doi.org/10.1007/978-1-4939-2438-7_21