Quantification of infectious bronchitis coronavirus by titration in vitro and in ovo

Joeri Kint*, Helena Jane Maier, Erik Jagt

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Quantification of the number of infectious viruses in a sample is a basic virological technique. In this chapter we provide a detailed description of three techniques to estimate the number of viable infectious avian coronaviruses in a sample. All three techniques are serial dilution assays, better known as titrations.

Original languageEnglish
Title of host publicationCoronaviruses: Methods and Protocols
PublisherSpringer New York LLC
Pages89-98
ISBN (Print)9781493924387, 9781493924370
DOIs
Publication statusPublished - 2015

Keywords

  • EID
  • Plaque-forming units
  • TCID
  • Titration

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  • Cite this

    Kint, J., Maier, H. J., & Jagt, E. (2015). Quantification of infectious bronchitis coronavirus by titration in vitro and in ovo. In Coronaviruses: Methods and Protocols (pp. 89-98). Springer New York LLC. https://doi.org/10.1007/978-1-4939-2438-7_9