Quantification of food polysaccharide mixtures by ¹H NMR

Donny W.H. Merkx, Yvonne Westphal, Ewoud J.J. van Velzen*, Kavish V. Thakoer, Niels de Roo, John P.M. van Duynhoven

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Polysaccharides are food ingredients that critically determine rheological properties and shelf life. A qualitative and quantitative assessment on food-specific polysaccharide mixtures by 1H NMR is presented. The method is based on the identification of intact polysaccharides, combined with a quantitative analysis of their monosaccharide constituents. Identification of the polysaccharides is achieved by 1H NMR line shape fitting with pure compound spectra. The monomeric composition was determined using the Saeman hydrolysis procedure, followed by direct monosaccharide quantification by 1H NMR. In the quantification, both the monosaccharide degradation during hydrolysis, as well as a correction for the non-instantaneous polysaccharide dissolution were taken into account. These factors were particularly important for the quantification of pectins. The method showed overall good repeatability (RSDr = 4.1 ± 0.9%) and within-laboratory reproducibility (RSDR = 6.1 ± 1.4%) for various food polysaccharides. Polysaccharide mixtures were quantitatively resolved by a non-negative least squares estimation, using identified polysaccharides and their molar monosaccharide stoichiometry as prior knowledge. The accuracy and precision of the presented method make it applicable to a wide range of food polysaccharide mixtures with complex and overlapping 1H NMR spectra.

Original languageEnglish
Pages (from-to)379-385
JournalCarbohydrate Polymers
Volume179
DOIs
Publication statusPublished - 1 Jan 2018

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Polysaccharides
Nuclear magnetic resonance
Monosaccharides
Hydrolysis
Pectins
Chemical analysis
Stoichiometry
Dissolution
Degradation

Keywords

  • Food polysaccharides
  • Mixture analysis
  • Non-instantaneous monosaccharide release
  • qNMR
  • Saeman hydrolysis

Cite this

Merkx, Donny W.H. ; Westphal, Yvonne ; van Velzen, Ewoud J.J. ; Thakoer, Kavish V. ; de Roo, Niels ; van Duynhoven, John P.M. / Quantification of food polysaccharide mixtures by ¹H NMR. In: Carbohydrate Polymers. 2018 ; Vol. 179. pp. 379-385.
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abstract = "Polysaccharides are food ingredients that critically determine rheological properties and shelf life. A qualitative and quantitative assessment on food-specific polysaccharide mixtures by 1H NMR is presented. The method is based on the identification of intact polysaccharides, combined with a quantitative analysis of their monosaccharide constituents. Identification of the polysaccharides is achieved by 1H NMR line shape fitting with pure compound spectra. The monomeric composition was determined using the Saeman hydrolysis procedure, followed by direct monosaccharide quantification by 1H NMR. In the quantification, both the monosaccharide degradation during hydrolysis, as well as a correction for the non-instantaneous polysaccharide dissolution were taken into account. These factors were particularly important for the quantification of pectins. The method showed overall good repeatability (RSDr = 4.1 ± 0.9{\%}) and within-laboratory reproducibility (RSDR = 6.1 ± 1.4{\%}) for various food polysaccharides. Polysaccharide mixtures were quantitatively resolved by a non-negative least squares estimation, using identified polysaccharides and their molar monosaccharide stoichiometry as prior knowledge. The accuracy and precision of the presented method make it applicable to a wide range of food polysaccharide mixtures with complex and overlapping 1H NMR spectra.",
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Quantification of food polysaccharide mixtures by ¹H NMR. / Merkx, Donny W.H.; Westphal, Yvonne; van Velzen, Ewoud J.J.; Thakoer, Kavish V.; de Roo, Niels; van Duynhoven, John P.M.

In: Carbohydrate Polymers, Vol. 179, 01.01.2018, p. 379-385.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Quantification of food polysaccharide mixtures by ¹H NMR

AU - Merkx, Donny W.H.

AU - Westphal, Yvonne

AU - van Velzen, Ewoud J.J.

AU - Thakoer, Kavish V.

AU - de Roo, Niels

AU - van Duynhoven, John P.M.

PY - 2018/1/1

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N2 - Polysaccharides are food ingredients that critically determine rheological properties and shelf life. A qualitative and quantitative assessment on food-specific polysaccharide mixtures by 1H NMR is presented. The method is based on the identification of intact polysaccharides, combined with a quantitative analysis of their monosaccharide constituents. Identification of the polysaccharides is achieved by 1H NMR line shape fitting with pure compound spectra. The monomeric composition was determined using the Saeman hydrolysis procedure, followed by direct monosaccharide quantification by 1H NMR. In the quantification, both the monosaccharide degradation during hydrolysis, as well as a correction for the non-instantaneous polysaccharide dissolution were taken into account. These factors were particularly important for the quantification of pectins. The method showed overall good repeatability (RSDr = 4.1 ± 0.9%) and within-laboratory reproducibility (RSDR = 6.1 ± 1.4%) for various food polysaccharides. Polysaccharide mixtures were quantitatively resolved by a non-negative least squares estimation, using identified polysaccharides and their molar monosaccharide stoichiometry as prior knowledge. The accuracy and precision of the presented method make it applicable to a wide range of food polysaccharide mixtures with complex and overlapping 1H NMR spectra.

AB - Polysaccharides are food ingredients that critically determine rheological properties and shelf life. A qualitative and quantitative assessment on food-specific polysaccharide mixtures by 1H NMR is presented. The method is based on the identification of intact polysaccharides, combined with a quantitative analysis of their monosaccharide constituents. Identification of the polysaccharides is achieved by 1H NMR line shape fitting with pure compound spectra. The monomeric composition was determined using the Saeman hydrolysis procedure, followed by direct monosaccharide quantification by 1H NMR. In the quantification, both the monosaccharide degradation during hydrolysis, as well as a correction for the non-instantaneous polysaccharide dissolution were taken into account. These factors were particularly important for the quantification of pectins. The method showed overall good repeatability (RSDr = 4.1 ± 0.9%) and within-laboratory reproducibility (RSDR = 6.1 ± 1.4%) for various food polysaccharides. Polysaccharide mixtures were quantitatively resolved by a non-negative least squares estimation, using identified polysaccharides and their molar monosaccharide stoichiometry as prior knowledge. The accuracy and precision of the presented method make it applicable to a wide range of food polysaccharide mixtures with complex and overlapping 1H NMR spectra.

KW - Food polysaccharides

KW - Mixture analysis

KW - Non-instantaneous monosaccharide release

KW - qNMR

KW - Saeman hydrolysis

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