Quantification of Celiac Disease Gluten Epitopes

Research output: Contribution to journalComment/Letter to the editorAcademic

Abstract

Scientists have published a novel method in the Journal of Chromatography A to detect multiple celiac disease-epitopes in wheat extracts simultaneously using liquid chromatography–multiple reaction monitoring mass spectrometry (LC–MRM–MS).1 Celiac disease is caused by an immune response in the small intestine from eating gluten proteins. An enzyme-linked immunosorbent assay (ELISA) is the standard testing method for food, but this can be inaccurate and does not differentiate between gluten proteins. Lead author Hetty van den Broeck told The Column: “This newly developed method based on mass spectrometry quantifies specifically celiac disease stimulating epitopes. This is not possible by ELISA, which detects gluten proteins in general by using a monoclonal antibody. Mass spectrometry allows specific detection and quantification of multiple CD-epitopes at the same time. This is necessary because of the complex genome of bread wheat and the many different CD-epitopes to which CD-patients can respond differentially.” In the current study, gluten extracts were prepared from wheat and extracted prior to LC–MRM–MS analysis. The authors developed the method to detect peptides known to induce the strongest immune response, as well as peptides with amino acid substitutions that make them harmless. The method was able to quantify different peptide isoforms, showing that a modern wheat variety, Toronto, contained the highest level of CD immunogenic peptides. The team are continuing their work to test and select reduced immunogenic wheat varieties, van den Broeck told The Column. She said: “We are also working on the development of screening body fluids from CD-patients for the presence of these epitopes or other specific peptide biomarkers for CD.”— B.D
LanguageEnglish
Pages6
JournalThe Column : the interactive e-publication for the global separation science industry
Volume11
Issue number11
Publication statusPublished - 2015

Fingerprint

Glutens
Epitopes
Mass spectrometry
Peptides
Immunosorbents
Liquid chromatography
Assays
Monitoring
Body fluids
Biomarkers
Enzymes
Chromatography
Screening
Protein Isoforms
Substitution reactions
Genes
Monoclonal Antibodies
Amino Acids
Testing

Cite this

@article{c3a13179cd1d4993bfaa4686bcffd5c0,
title = "Quantification of Celiac Disease Gluten Epitopes",
abstract = "Scientists have published a novel method in the Journal of Chromatography A to detect multiple celiac disease-epitopes in wheat extracts simultaneously using liquid chromatography–multiple reaction monitoring mass spectrometry (LC–MRM–MS).1 Celiac disease is caused by an immune response in the small intestine from eating gluten proteins. An enzyme-linked immunosorbent assay (ELISA) is the standard testing method for food, but this can be inaccurate and does not differentiate between gluten proteins. Lead author Hetty van den Broeck told The Column: “This newly developed method based on mass spectrometry quantifies specifically celiac disease stimulating epitopes. This is not possible by ELISA, which detects gluten proteins in general by using a monoclonal antibody. Mass spectrometry allows specific detection and quantification of multiple CD-epitopes at the same time. This is necessary because of the complex genome of bread wheat and the many different CD-epitopes to which CD-patients can respond differentially.” In the current study, gluten extracts were prepared from wheat and extracted prior to LC–MRM–MS analysis. The authors developed the method to detect peptides known to induce the strongest immune response, as well as peptides with amino acid substitutions that make them harmless. The method was able to quantify different peptide isoforms, showing that a modern wheat variety, Toronto, contained the highest level of CD immunogenic peptides. The team are continuing their work to test and select reduced immunogenic wheat varieties, van den Broeck told The Column. She said: “We are also working on the development of screening body fluids from CD-patients for the presence of these epitopes or other specific peptide biomarkers for CD.”— B.D",
author = "B. Degg and {Busink-van den Broeck}, H.C.",
note = "http://www.chromatographyonline.com/quantification-celiac-disease-gluten-epitopes",
year = "2015",
language = "English",
volume = "11",
pages = "6",
journal = "The Column : the interactive e-publication for the global separation science industry",
issn = "2050-280X",
number = "11",

}

Quantification of Celiac Disease Gluten Epitopes. / Degg, B.; Busink-van den Broeck, H.C.

In: The Column : the interactive e-publication for the global separation science industry, Vol. 11, No. 11, 2015, p. 6.

Research output: Contribution to journalComment/Letter to the editorAcademic

TY - JOUR

T1 - Quantification of Celiac Disease Gluten Epitopes

AU - Degg, B.

AU - Busink-van den Broeck, H.C.

N1 - http://www.chromatographyonline.com/quantification-celiac-disease-gluten-epitopes

PY - 2015

Y1 - 2015

N2 - Scientists have published a novel method in the Journal of Chromatography A to detect multiple celiac disease-epitopes in wheat extracts simultaneously using liquid chromatography–multiple reaction monitoring mass spectrometry (LC–MRM–MS).1 Celiac disease is caused by an immune response in the small intestine from eating gluten proteins. An enzyme-linked immunosorbent assay (ELISA) is the standard testing method for food, but this can be inaccurate and does not differentiate between gluten proteins. Lead author Hetty van den Broeck told The Column: “This newly developed method based on mass spectrometry quantifies specifically celiac disease stimulating epitopes. This is not possible by ELISA, which detects gluten proteins in general by using a monoclonal antibody. Mass spectrometry allows specific detection and quantification of multiple CD-epitopes at the same time. This is necessary because of the complex genome of bread wheat and the many different CD-epitopes to which CD-patients can respond differentially.” In the current study, gluten extracts were prepared from wheat and extracted prior to LC–MRM–MS analysis. The authors developed the method to detect peptides known to induce the strongest immune response, as well as peptides with amino acid substitutions that make them harmless. The method was able to quantify different peptide isoforms, showing that a modern wheat variety, Toronto, contained the highest level of CD immunogenic peptides. The team are continuing their work to test and select reduced immunogenic wheat varieties, van den Broeck told The Column. She said: “We are also working on the development of screening body fluids from CD-patients for the presence of these epitopes or other specific peptide biomarkers for CD.”— B.D

AB - Scientists have published a novel method in the Journal of Chromatography A to detect multiple celiac disease-epitopes in wheat extracts simultaneously using liquid chromatography–multiple reaction monitoring mass spectrometry (LC–MRM–MS).1 Celiac disease is caused by an immune response in the small intestine from eating gluten proteins. An enzyme-linked immunosorbent assay (ELISA) is the standard testing method for food, but this can be inaccurate and does not differentiate between gluten proteins. Lead author Hetty van den Broeck told The Column: “This newly developed method based on mass spectrometry quantifies specifically celiac disease stimulating epitopes. This is not possible by ELISA, which detects gluten proteins in general by using a monoclonal antibody. Mass spectrometry allows specific detection and quantification of multiple CD-epitopes at the same time. This is necessary because of the complex genome of bread wheat and the many different CD-epitopes to which CD-patients can respond differentially.” In the current study, gluten extracts were prepared from wheat and extracted prior to LC–MRM–MS analysis. The authors developed the method to detect peptides known to induce the strongest immune response, as well as peptides with amino acid substitutions that make them harmless. The method was able to quantify different peptide isoforms, showing that a modern wheat variety, Toronto, contained the highest level of CD immunogenic peptides. The team are continuing their work to test and select reduced immunogenic wheat varieties, van den Broeck told The Column. She said: “We are also working on the development of screening body fluids from CD-patients for the presence of these epitopes or other specific peptide biomarkers for CD.”— B.D

M3 - Comment/Letter to the editor

VL - 11

SP - 6

JO - The Column : the interactive e-publication for the global separation science industry

T2 - The Column : the interactive e-publication for the global separation science industry

JF - The Column : the interactive e-publication for the global separation science industry

SN - 2050-280X

IS - 11

ER -