Quality of porcine blastocysts produced in vitro in the presence of absence of GH

A. Kidson, F.J. Rubio-Pomar, A. van Knegsel, H.T.A. van Tol, W. Hazeleger, D.W.B. Ducro-Steverink, B. Colenbrander, S.J. Dieleman, M.M. Bevers

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

GH receptor (GHR) mRNA is expressed in bovine in vitro produced embryos up to the blastocyst stage and GH improves the quality of bovine embryos by increasing blastocyst cell numbers and reducing the incidence of apoptosis as evaluated by DNA strand-break labelling. Porcine in vitro produced blastocysts have lower cell numbers than in vivo blastocysts and exhibit higher incidences of apoptosis. Therefore we investigated the effects of 100 ng GH/ml NCSU23 medium during in vitro culture of presumptive in vitro fertilized sow zygotes on embryo development and blastocyst quality (defined by diameter, cell number, apoptosis and survival after non-surgical transfer). In vivo produced blastocysts were analysed concurrently as a reference value. GHR was expressed in embryos from the 2-cell to blastocyst stages. GH had no effect on blastocyst development or cell numbers, but increased the mean blastocyst diameter. The incidence of apoptosis, detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL), was decreased by GH, but when non-TUNEL-labelled apoptotic fragmented nuclei were included, no difference was seen. GH appeared to slow down the progression of apoptosis though. In vivo produced blastocysts presented no apoptotic nuclei, and contained higher cell numbers and larger diameters. Pregnancy rates on day 11 were similar for all groups, but survival was poorer for in vitro than in vivo produced blastocysts. In this study GH appeared to be beneficial only from the blastocyst stage, but the presence of GHR from early cleavage stages nevertheless indicates a role for GH throughout porcine embryo development and deserves further investigation.
Original languageEnglish
Pages (from-to)165-177
JournalReproduction
Volume127
DOIs
Publication statusPublished - 2004

Fingerprint

Blastocyst
Swine
Cell Count
Apoptosis
Embryonic Structures
Embryonic Development
In Vitro Techniques
Incidence
DNA Breaks
DNA Nucleotidylexotransferase
Zygote
Pregnancy Rate
Cell Survival
Reference Values

Keywords

  • growth-hormone receptor
  • preimplantation embryo development
  • inner cell mass
  • bovine embryos
  • dna fragmentation
  • pig embryo
  • zygotic transition
  • inhibits apoptosis
  • mouse blastocyst
  • rat blastocysts

Cite this

Kidson, A., Rubio-Pomar, F. J., van Knegsel, A., van Tol, H. T. A., Hazeleger, W., Ducro-Steverink, D. W. B., ... Bevers, M. M. (2004). Quality of porcine blastocysts produced in vitro in the presence of absence of GH. Reproduction, 127, 165-177. https://doi.org/10.1530/rep.1.00086
Kidson, A. ; Rubio-Pomar, F.J. ; van Knegsel, A. ; van Tol, H.T.A. ; Hazeleger, W. ; Ducro-Steverink, D.W.B. ; Colenbrander, B. ; Dieleman, S.J. ; Bevers, M.M. / Quality of porcine blastocysts produced in vitro in the presence of absence of GH. In: Reproduction. 2004 ; Vol. 127. pp. 165-177.
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abstract = "GH receptor (GHR) mRNA is expressed in bovine in vitro produced embryos up to the blastocyst stage and GH improves the quality of bovine embryos by increasing blastocyst cell numbers and reducing the incidence of apoptosis as evaluated by DNA strand-break labelling. Porcine in vitro produced blastocysts have lower cell numbers than in vivo blastocysts and exhibit higher incidences of apoptosis. Therefore we investigated the effects of 100 ng GH/ml NCSU23 medium during in vitro culture of presumptive in vitro fertilized sow zygotes on embryo development and blastocyst quality (defined by diameter, cell number, apoptosis and survival after non-surgical transfer). In vivo produced blastocysts were analysed concurrently as a reference value. GHR was expressed in embryos from the 2-cell to blastocyst stages. GH had no effect on blastocyst development or cell numbers, but increased the mean blastocyst diameter. The incidence of apoptosis, detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL), was decreased by GH, but when non-TUNEL-labelled apoptotic fragmented nuclei were included, no difference was seen. GH appeared to slow down the progression of apoptosis though. In vivo produced blastocysts presented no apoptotic nuclei, and contained higher cell numbers and larger diameters. Pregnancy rates on day 11 were similar for all groups, but survival was poorer for in vitro than in vivo produced blastocysts. In this study GH appeared to be beneficial only from the blastocyst stage, but the presence of GHR from early cleavage stages nevertheless indicates a role for GH throughout porcine embryo development and deserves further investigation.",
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Kidson, A, Rubio-Pomar, FJ, van Knegsel, A, van Tol, HTA, Hazeleger, W, Ducro-Steverink, DWB, Colenbrander, B, Dieleman, SJ & Bevers, MM 2004, 'Quality of porcine blastocysts produced in vitro in the presence of absence of GH', Reproduction, vol. 127, pp. 165-177. https://doi.org/10.1530/rep.1.00086

Quality of porcine blastocysts produced in vitro in the presence of absence of GH. / Kidson, A.; Rubio-Pomar, F.J.; van Knegsel, A.; van Tol, H.T.A.; Hazeleger, W.; Ducro-Steverink, D.W.B.; Colenbrander, B.; Dieleman, S.J.; Bevers, M.M.

In: Reproduction, Vol. 127, 2004, p. 165-177.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Quality of porcine blastocysts produced in vitro in the presence of absence of GH

AU - Kidson, A.

AU - Rubio-Pomar, F.J.

AU - van Knegsel, A.

AU - van Tol, H.T.A.

AU - Hazeleger, W.

AU - Ducro-Steverink, D.W.B.

AU - Colenbrander, B.

AU - Dieleman, S.J.

AU - Bevers, M.M.

PY - 2004

Y1 - 2004

N2 - GH receptor (GHR) mRNA is expressed in bovine in vitro produced embryos up to the blastocyst stage and GH improves the quality of bovine embryos by increasing blastocyst cell numbers and reducing the incidence of apoptosis as evaluated by DNA strand-break labelling. Porcine in vitro produced blastocysts have lower cell numbers than in vivo blastocysts and exhibit higher incidences of apoptosis. Therefore we investigated the effects of 100 ng GH/ml NCSU23 medium during in vitro culture of presumptive in vitro fertilized sow zygotes on embryo development and blastocyst quality (defined by diameter, cell number, apoptosis and survival after non-surgical transfer). In vivo produced blastocysts were analysed concurrently as a reference value. GHR was expressed in embryos from the 2-cell to blastocyst stages. GH had no effect on blastocyst development or cell numbers, but increased the mean blastocyst diameter. The incidence of apoptosis, detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL), was decreased by GH, but when non-TUNEL-labelled apoptotic fragmented nuclei were included, no difference was seen. GH appeared to slow down the progression of apoptosis though. In vivo produced blastocysts presented no apoptotic nuclei, and contained higher cell numbers and larger diameters. Pregnancy rates on day 11 were similar for all groups, but survival was poorer for in vitro than in vivo produced blastocysts. In this study GH appeared to be beneficial only from the blastocyst stage, but the presence of GHR from early cleavage stages nevertheless indicates a role for GH throughout porcine embryo development and deserves further investigation.

AB - GH receptor (GHR) mRNA is expressed in bovine in vitro produced embryos up to the blastocyst stage and GH improves the quality of bovine embryos by increasing blastocyst cell numbers and reducing the incidence of apoptosis as evaluated by DNA strand-break labelling. Porcine in vitro produced blastocysts have lower cell numbers than in vivo blastocysts and exhibit higher incidences of apoptosis. Therefore we investigated the effects of 100 ng GH/ml NCSU23 medium during in vitro culture of presumptive in vitro fertilized sow zygotes on embryo development and blastocyst quality (defined by diameter, cell number, apoptosis and survival after non-surgical transfer). In vivo produced blastocysts were analysed concurrently as a reference value. GHR was expressed in embryos from the 2-cell to blastocyst stages. GH had no effect on blastocyst development or cell numbers, but increased the mean blastocyst diameter. The incidence of apoptosis, detected by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL), was decreased by GH, but when non-TUNEL-labelled apoptotic fragmented nuclei were included, no difference was seen. GH appeared to slow down the progression of apoptosis though. In vivo produced blastocysts presented no apoptotic nuclei, and contained higher cell numbers and larger diameters. Pregnancy rates on day 11 were similar for all groups, but survival was poorer for in vitro than in vivo produced blastocysts. In this study GH appeared to be beneficial only from the blastocyst stage, but the presence of GHR from early cleavage stages nevertheless indicates a role for GH throughout porcine embryo development and deserves further investigation.

KW - growth-hormone receptor

KW - preimplantation embryo development

KW - inner cell mass

KW - bovine embryos

KW - dna fragmentation

KW - pig embryo

KW - zygotic transition

KW - inhibits apoptosis

KW - mouse blastocyst

KW - rat blastocysts

U2 - 10.1530/rep.1.00086

DO - 10.1530/rep.1.00086

M3 - Article

VL - 127

SP - 165

EP - 177

JO - Reproduction

JF - Reproduction

SN - 1470-1626

ER -

Kidson A, Rubio-Pomar FJ, van Knegsel A, van Tol HTA, Hazeleger W, Ducro-Steverink DWB et al. Quality of porcine blastocysts produced in vitro in the presence of absence of GH. Reproduction. 2004;127:165-177. https://doi.org/10.1530/rep.1.00086