QTL mapping of thrips resistance in pepper

A. Maharijaya, B.J. Vosman, M.M. Steenhuis-Broers, K.T.B. Pelgrom, A. Purwito, R.G.F. Visser, R.E. Voorrips

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Thrips is one of the most damaging pests in pepper (Capsicum). Resistance to thrips was identified in Capsicum annuum. This study was aimed at the elucidation of the genetic background of thrips resistance in Capsicum through QTL mapping. The QTL analysis was carried out for Frankliniella occidentalis resistance in an F2 population consisting of 196 plants derived from an interspecific cross between the highly resistant C. annuum AC 1979 as female parent and the highly susceptible C. chinense 4661 as male parent. Fifty-seven SSR, 109 AFLP, and 5 SNP markers were used to construct a genetic map with a total length of 1636 cM. Damage caused by larvae and the survival of first and second instar larval stages observed in a no-choice test were used as parameters of resistance. Interval mapping detected one QTL for each of these parameters, all co-localizing near the same marker on chromosome 6. Use of this marker as co-factor in a multiple-QTL mapping analysis failed to uncover any additional QTLs. This QTL explained about 50 % of the genetic variation, and the resistance allele of this QTL was inherited from the resistant parent. Thrips resistance was not linked to trichome density.
Original languageEnglish
Pages (from-to)1945-1956
JournalTheoretical and Applied Genetics
Volume128
Issue number10
DOIs
Publication statusPublished - 2015

Fingerprint

Thysanoptera
Capsicum
sweet peppers
quantitative trait loci
Thrips (Thripidae)
Trichomes
Chromosomes, Human, Pair 6
Capsicum annuum
Single Nucleotide Polymorphism
Larva
Alleles
Frankliniella occidentalis
trichomes
genetic background
amplified fragment length polymorphism
Population
instars
pests
alleles
chromosomes

Cite this

Maharijaya, A. ; Vosman, B.J. ; Steenhuis-Broers, M.M. ; Pelgrom, K.T.B. ; Purwito, A. ; Visser, R.G.F. ; Voorrips, R.E. / QTL mapping of thrips resistance in pepper. In: Theoretical and Applied Genetics. 2015 ; Vol. 128, No. 10. pp. 1945-1956.
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abstract = "Thrips is one of the most damaging pests in pepper (Capsicum). Resistance to thrips was identified in Capsicum annuum. This study was aimed at the elucidation of the genetic background of thrips resistance in Capsicum through QTL mapping. The QTL analysis was carried out for Frankliniella occidentalis resistance in an F2 population consisting of 196 plants derived from an interspecific cross between the highly resistant C. annuum AC 1979 as female parent and the highly susceptible C. chinense 4661 as male parent. Fifty-seven SSR, 109 AFLP, and 5 SNP markers were used to construct a genetic map with a total length of 1636 cM. Damage caused by larvae and the survival of first and second instar larval stages observed in a no-choice test were used as parameters of resistance. Interval mapping detected one QTL for each of these parameters, all co-localizing near the same marker on chromosome 6. Use of this marker as co-factor in a multiple-QTL mapping analysis failed to uncover any additional QTLs. This QTL explained about 50 {\%} of the genetic variation, and the resistance allele of this QTL was inherited from the resistant parent. Thrips resistance was not linked to trichome density.",
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Maharijaya, A, Vosman, BJ, Steenhuis-Broers, MM, Pelgrom, KTB, Purwito, A, Visser, RGF & Voorrips, RE 2015, 'QTL mapping of thrips resistance in pepper', Theoretical and Applied Genetics, vol. 128, no. 10, pp. 1945-1956. https://doi.org/10.1007/s00122-015-2558-1

QTL mapping of thrips resistance in pepper. / Maharijaya, A.; Vosman, B.J.; Steenhuis-Broers, M.M.; Pelgrom, K.T.B.; Purwito, A.; Visser, R.G.F.; Voorrips, R.E.

In: Theoretical and Applied Genetics, Vol. 128, No. 10, 2015, p. 1945-1956.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Maharijaya, A.

AU - Vosman, B.J.

AU - Steenhuis-Broers, M.M.

AU - Pelgrom, K.T.B.

AU - Purwito, A.

AU - Visser, R.G.F.

AU - Voorrips, R.E.

PY - 2015

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AB - Thrips is one of the most damaging pests in pepper (Capsicum). Resistance to thrips was identified in Capsicum annuum. This study was aimed at the elucidation of the genetic background of thrips resistance in Capsicum through QTL mapping. The QTL analysis was carried out for Frankliniella occidentalis resistance in an F2 population consisting of 196 plants derived from an interspecific cross between the highly resistant C. annuum AC 1979 as female parent and the highly susceptible C. chinense 4661 as male parent. Fifty-seven SSR, 109 AFLP, and 5 SNP markers were used to construct a genetic map with a total length of 1636 cM. Damage caused by larvae and the survival of first and second instar larval stages observed in a no-choice test were used as parameters of resistance. Interval mapping detected one QTL for each of these parameters, all co-localizing near the same marker on chromosome 6. Use of this marker as co-factor in a multiple-QTL mapping analysis failed to uncover any additional QTLs. This QTL explained about 50 % of the genetic variation, and the resistance allele of this QTL was inherited from the resistant parent. Thrips resistance was not linked to trichome density.

U2 - 10.1007/s00122-015-2558-1

DO - 10.1007/s00122-015-2558-1

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JO - Theoretical and Applied Genetics

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SN - 0040-5752

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Maharijaya A, Vosman BJ, Steenhuis-Broers MM, Pelgrom KTB, Purwito A, Visser RGF et al. QTL mapping of thrips resistance in pepper. Theoretical and Applied Genetics. 2015;128(10):1945-1956. https://doi.org/10.1007/s00122-015-2558-1