QTL mapping in diploid potato by using selfed progenies of the cross S. tuberosum × S. chacoense

D. Meijer, M. Viquez-Zamora, H.J. van Eck, R.C.B. Hutten, Y. Su, R. Rothengatter, R.G.F. Visser, W.H. Lindhout, A.W. van Heusden*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Usually, mapping studies in potato are performed with segregating populations from crosses between highly heterozygous diploid or tetraploid parents. These studies are hampered by a high level of genetic background noise due to the numerous segregating alleles, with a maximum of eight per locus. In the present study, we aimed to increase the mapping efficiency by using progenies from diploid inbred populations in which at most two alleles segregate. Selfed progenies were generated from a cross between S. tuberosum (D2; a highly heterozygous diploid) and S. chacoense (DS; a homozygous diploid clone) containing the self-incompatibility overcoming S locus inhibitor (Sli-gene). The Sli-gene enables self-pollination and the generation of selfed progenies. One F2 population was used to map several quality traits, such as tuber shape, flesh and skin color. Quantitative trait loci were identified for almost all traits under investigation. The identified loci partially coincided with known mapped loci and partially identified new loci. Nine F3 populations were used to validate the QTLs and monitor the overall increase in the homozygosity level.

Original languageEnglish
Article number121
JournalEuphytica
Volume214
Issue number7
DOIs
Publication statusPublished - Jul 2018

Fingerprint

Solanum tuberosum
Diploidy
quantitative trait loci
diploidy
potatoes
loci
Population
Alleles
Skin Pigmentation
Pollination
Tetraploidy
Quantitative Trait Loci
alleles
Genes
self-pollination
Noise
homozygosity
Clone Cells
genetic background
tetraploidy

Keywords

  • Diploid potato
  • Homozygosity
  • Inbreeding
  • Self-compatibility

Cite this

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title = "QTL mapping in diploid potato by using selfed progenies of the cross S. tuberosum × S. chacoense",
abstract = "Usually, mapping studies in potato are performed with segregating populations from crosses between highly heterozygous diploid or tetraploid parents. These studies are hampered by a high level of genetic background noise due to the numerous segregating alleles, with a maximum of eight per locus. In the present study, we aimed to increase the mapping efficiency by using progenies from diploid inbred populations in which at most two alleles segregate. Selfed progenies were generated from a cross between S. tuberosum (D2; a highly heterozygous diploid) and S. chacoense (DS; a homozygous diploid clone) containing the self-incompatibility overcoming S locus inhibitor (Sli-gene). The Sli-gene enables self-pollination and the generation of selfed progenies. One F2 population was used to map several quality traits, such as tuber shape, flesh and skin color. Quantitative trait loci were identified for almost all traits under investigation. The identified loci partially coincided with known mapped loci and partially identified new loci. Nine F3 populations were used to validate the QTLs and monitor the overall increase in the homozygosity level.",
keywords = "Diploid potato, Homozygosity, Inbreeding, Self-compatibility",
author = "D. Meijer and M. Viquez-Zamora and {van Eck}, H.J. and R.C.B. Hutten and Y. Su and R. Rothengatter and R.G.F. Visser and W.H. Lindhout and {van Heusden}, A.W.",
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language = "English",
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QTL mapping in diploid potato by using selfed progenies of the cross S. tuberosum × S. chacoense. / Meijer, D.; Viquez-Zamora, M.; van Eck, H.J.; Hutten, R.C.B.; Su, Y.; Rothengatter, R.; Visser, R.G.F.; Lindhout, W.H.; van Heusden, A.W.

In: Euphytica, Vol. 214, No. 7, 121, 07.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - QTL mapping in diploid potato by using selfed progenies of the cross S. tuberosum × S. chacoense

AU - Meijer, D.

AU - Viquez-Zamora, M.

AU - van Eck, H.J.

AU - Hutten, R.C.B.

AU - Su, Y.

AU - Rothengatter, R.

AU - Visser, R.G.F.

AU - Lindhout, W.H.

AU - van Heusden, A.W.

PY - 2018/7

Y1 - 2018/7

N2 - Usually, mapping studies in potato are performed with segregating populations from crosses between highly heterozygous diploid or tetraploid parents. These studies are hampered by a high level of genetic background noise due to the numerous segregating alleles, with a maximum of eight per locus. In the present study, we aimed to increase the mapping efficiency by using progenies from diploid inbred populations in which at most two alleles segregate. Selfed progenies were generated from a cross between S. tuberosum (D2; a highly heterozygous diploid) and S. chacoense (DS; a homozygous diploid clone) containing the self-incompatibility overcoming S locus inhibitor (Sli-gene). The Sli-gene enables self-pollination and the generation of selfed progenies. One F2 population was used to map several quality traits, such as tuber shape, flesh and skin color. Quantitative trait loci were identified for almost all traits under investigation. The identified loci partially coincided with known mapped loci and partially identified new loci. Nine F3 populations were used to validate the QTLs and monitor the overall increase in the homozygosity level.

AB - Usually, mapping studies in potato are performed with segregating populations from crosses between highly heterozygous diploid or tetraploid parents. These studies are hampered by a high level of genetic background noise due to the numerous segregating alleles, with a maximum of eight per locus. In the present study, we aimed to increase the mapping efficiency by using progenies from diploid inbred populations in which at most two alleles segregate. Selfed progenies were generated from a cross between S. tuberosum (D2; a highly heterozygous diploid) and S. chacoense (DS; a homozygous diploid clone) containing the self-incompatibility overcoming S locus inhibitor (Sli-gene). The Sli-gene enables self-pollination and the generation of selfed progenies. One F2 population was used to map several quality traits, such as tuber shape, flesh and skin color. Quantitative trait loci were identified for almost all traits under investigation. The identified loci partially coincided with known mapped loci and partially identified new loci. Nine F3 populations were used to validate the QTLs and monitor the overall increase in the homozygosity level.

KW - Diploid potato

KW - Homozygosity

KW - Inbreeding

KW - Self-compatibility

U2 - 10.1007/s10681-018-2191-6

DO - 10.1007/s10681-018-2191-6

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VL - 214

JO - Euphytica

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SN - 0014-2336

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