QTL analysis of the genetic architecture determining resistance to fire blight in an apple progeny

F. Calenge, D. Drouet, W.E. van de Weg, M.N. Brisset, J.P. Paulin, C.E. Durel

    Research output: Contribution to journalArticleAcademicpeer-review

    2 Citations (Scopus)

    Abstract

    Fire blight, caused by the bacterial pathogen Erwinia amylovora, is one of the most destructive diseases of apple (Malus x domestica). In order to analyse the genetic determinism of resistance to fire blight in apple, a quantitative trait analysis (QTL) approach was used. A F1 progeny of 164 individuals derived from a cross between the apple cultivars `Prima¿ and `Fiesta¿ was inoculated in greenhouse conditions. Seven copies per genotype were used. The length of the necrosis observed on shoots was scored 7 and 14 days after inoculation. The MapQTL software was used for QTL analyses, using two previously built maps of the parents, and the symptoms scored on shoots. Digenic interactions between all pairwise combinations of genetic markers were tested using a two-way ANOVA model with the SAS software. QTL were detected at the same locations both 7 and 14 days after inoculation. Two weak effect QTL deriving from `Prima¿ were detected on linkage groups (LG) 3 and LG16. One strong effect QTL deriving from `Fiesta¿ was detected on LG7 that explained 46.6% of the phenotypic variation observed in the progeny. Two additional significant (P
    LanguageEnglish
    Pages141-146
    JournalActa Horticulturae
    Volume663
    DOIs
    Publication statusPublished - 2004

    Fingerprint

    Erwinia amylovora
    quantitative traits
    genetic techniques and protocols
    apples
    shoots
    Malus
    genetic resistance
    linkage groups
    phenotypic variation
    signs and symptoms (plants)
    necrosis
    analysis of variance
    greenhouses
    genetic markers
    pathogens
    genotype
    cultivars

    Cite this

    Calenge, F. ; Drouet, D. ; van de Weg, W.E. ; Brisset, M.N. ; Paulin, J.P. ; Durel, C.E. / QTL analysis of the genetic architecture determining resistance to fire blight in an apple progeny. In: Acta Horticulturae. 2004 ; Vol. 663. pp. 141-146.
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    title = "QTL analysis of the genetic architecture determining resistance to fire blight in an apple progeny",
    abstract = "Fire blight, caused by the bacterial pathogen Erwinia amylovora, is one of the most destructive diseases of apple (Malus x domestica). In order to analyse the genetic determinism of resistance to fire blight in apple, a quantitative trait analysis (QTL) approach was used. A F1 progeny of 164 individuals derived from a cross between the apple cultivars `Prima¿ and `Fiesta¿ was inoculated in greenhouse conditions. Seven copies per genotype were used. The length of the necrosis observed on shoots was scored 7 and 14 days after inoculation. The MapQTL software was used for QTL analyses, using two previously built maps of the parents, and the symptoms scored on shoots. Digenic interactions between all pairwise combinations of genetic markers were tested using a two-way ANOVA model with the SAS software. QTL were detected at the same locations both 7 and 14 days after inoculation. Two weak effect QTL deriving from `Prima¿ were detected on linkage groups (LG) 3 and LG16. One strong effect QTL deriving from `Fiesta¿ was detected on LG7 that explained 46.6{\%} of the phenotypic variation observed in the progeny. Two additional significant (P",
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    QTL analysis of the genetic architecture determining resistance to fire blight in an apple progeny. / Calenge, F.; Drouet, D.; van de Weg, W.E.; Brisset, M.N.; Paulin, J.P.; Durel, C.E.

    In: Acta Horticulturae, Vol. 663, 2004, p. 141-146.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - van de Weg, W.E.

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    AU - Paulin, J.P.

    AU - Durel, C.E.

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