QTL analysis for stomatal functioning in tetraploid Rosa x hybrida grown at high relative air humidity and its implications on postharvest longevity

D.R.A. Carvalho, C.F.S. Koning, D. Fanourakis, M.W. Vasconcelos, S.M.P. Carvalho, E. Heuvelink, F.A. Krens, C.A. Maliepaard

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

High relative air humidity (RH = 85 %) during leaf development disturbs stomatal functioning leading to excessive water loss in conditions of high evaporative demand, resulting in severe reduction in postharvest longevity. In roses, this effect depends on the genotype, opening the possibility for breeding cultivars with more responsive stomata. In this study, we aim at identifying genomic regions associated with the control of water loss following growth at high RH. The F1 generation (108 offspring) and the two parents (P540 and P867) of a tetraploid cut rose population grown at high (85 %) RH were phenotyped for stomatal control to water loss by assessing the relative water content after 4 h of leaflet desiccation (RWC_4 h). The RWC_4 h varied between 7 and 62 % across the 110 studied individuals, with parents P540 and P867 showing 51 and 20 % RWC_4 h, respectively. Based on these data, a quantitative trait locus (QTL) analysis was performed. The impact of the identified QTLs on postharvest longevity of ten selected offspring was further evaluated. Three QTLs were identified: two major [positioned on linkage group 5 of the integrated consensus map (ICM 5) of both parents and on ICM 2 of the parent P867] and one putative minor (mapped to ICM 6 of both parents), explaining 32 % of the variability in the RWC_4 h. Low RWC_4 h was found to be a good proxy for eliminating the offspring with short vase life. This study constitutes a first step toward identifying the most likely regions for genes of interest controlling stomatal functioning in high RH-grown plants.
Original languageEnglish
Article number172
Number of pages11
JournalMolecular Breeding
Volume35
DOIs
Publication statusPublished - 2015

Fingerprint

Tetraploidy
Quantitative Trait Loci
Humidity
tetraploidy
quantitative trait loci
humidity
Rosa
Parents
Air
air
Water
vase life
water
leaf development
desiccation (plant physiology)
linkage groups
Desiccation
stomata
Proxy
Breeding

Keywords

  • marker-assisted selection
  • water relations
  • cut roses
  • in-vitro
  • traits
  • environments
  • conductance
  • sensitivity
  • improvement
  • resistance

Cite this

@article{e81b3a8a0c9f48c4abfdb2eef5961543,
title = "QTL analysis for stomatal functioning in tetraploid Rosa x hybrida grown at high relative air humidity and its implications on postharvest longevity",
abstract = "High relative air humidity (RH = 85 {\%}) during leaf development disturbs stomatal functioning leading to excessive water loss in conditions of high evaporative demand, resulting in severe reduction in postharvest longevity. In roses, this effect depends on the genotype, opening the possibility for breeding cultivars with more responsive stomata. In this study, we aim at identifying genomic regions associated with the control of water loss following growth at high RH. The F1 generation (108 offspring) and the two parents (P540 and P867) of a tetraploid cut rose population grown at high (85 {\%}) RH were phenotyped for stomatal control to water loss by assessing the relative water content after 4 h of leaflet desiccation (RWC_4 h). The RWC_4 h varied between 7 and 62 {\%} across the 110 studied individuals, with parents P540 and P867 showing 51 and 20 {\%} RWC_4 h, respectively. Based on these data, a quantitative trait locus (QTL) analysis was performed. The impact of the identified QTLs on postharvest longevity of ten selected offspring was further evaluated. Three QTLs were identified: two major [positioned on linkage group 5 of the integrated consensus map (ICM 5) of both parents and on ICM 2 of the parent P867] and one putative minor (mapped to ICM 6 of both parents), explaining 32 {\%} of the variability in the RWC_4 h. Low RWC_4 h was found to be a good proxy for eliminating the offspring with short vase life. This study constitutes a first step toward identifying the most likely regions for genes of interest controlling stomatal functioning in high RH-grown plants.",
keywords = "marker-assisted selection, water relations, cut roses, in-vitro, traits, environments, conductance, sensitivity, improvement, resistance",
author = "D.R.A. Carvalho and C.F.S. Koning and D. Fanourakis and M.W. Vasconcelos and S.M.P. Carvalho and E. Heuvelink and F.A. Krens and C.A. Maliepaard",
year = "2015",
doi = "10.1007/s11032-015-0365-7",
language = "English",
volume = "35",
journal = "Molecular Breeding",
issn = "1380-3743",
publisher = "Springer Verlag",

}

QTL analysis for stomatal functioning in tetraploid Rosa x hybrida grown at high relative air humidity and its implications on postharvest longevity. / Carvalho, D.R.A.; Koning, C.F.S.; Fanourakis, D.; Vasconcelos, M.W.; Carvalho, S.M.P.; Heuvelink, E.; Krens, F.A.; Maliepaard, C.A.

In: Molecular Breeding, Vol. 35, 172, 2015.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - QTL analysis for stomatal functioning in tetraploid Rosa x hybrida grown at high relative air humidity and its implications on postharvest longevity

AU - Carvalho, D.R.A.

AU - Koning, C.F.S.

AU - Fanourakis, D.

AU - Vasconcelos, M.W.

AU - Carvalho, S.M.P.

AU - Heuvelink, E.

AU - Krens, F.A.

AU - Maliepaard, C.A.

PY - 2015

Y1 - 2015

N2 - High relative air humidity (RH = 85 %) during leaf development disturbs stomatal functioning leading to excessive water loss in conditions of high evaporative demand, resulting in severe reduction in postharvest longevity. In roses, this effect depends on the genotype, opening the possibility for breeding cultivars with more responsive stomata. In this study, we aim at identifying genomic regions associated with the control of water loss following growth at high RH. The F1 generation (108 offspring) and the two parents (P540 and P867) of a tetraploid cut rose population grown at high (85 %) RH were phenotyped for stomatal control to water loss by assessing the relative water content after 4 h of leaflet desiccation (RWC_4 h). The RWC_4 h varied between 7 and 62 % across the 110 studied individuals, with parents P540 and P867 showing 51 and 20 % RWC_4 h, respectively. Based on these data, a quantitative trait locus (QTL) analysis was performed. The impact of the identified QTLs on postharvest longevity of ten selected offspring was further evaluated. Three QTLs were identified: two major [positioned on linkage group 5 of the integrated consensus map (ICM 5) of both parents and on ICM 2 of the parent P867] and one putative minor (mapped to ICM 6 of both parents), explaining 32 % of the variability in the RWC_4 h. Low RWC_4 h was found to be a good proxy for eliminating the offspring with short vase life. This study constitutes a first step toward identifying the most likely regions for genes of interest controlling stomatal functioning in high RH-grown plants.

AB - High relative air humidity (RH = 85 %) during leaf development disturbs stomatal functioning leading to excessive water loss in conditions of high evaporative demand, resulting in severe reduction in postharvest longevity. In roses, this effect depends on the genotype, opening the possibility for breeding cultivars with more responsive stomata. In this study, we aim at identifying genomic regions associated with the control of water loss following growth at high RH. The F1 generation (108 offspring) and the two parents (P540 and P867) of a tetraploid cut rose population grown at high (85 %) RH were phenotyped for stomatal control to water loss by assessing the relative water content after 4 h of leaflet desiccation (RWC_4 h). The RWC_4 h varied between 7 and 62 % across the 110 studied individuals, with parents P540 and P867 showing 51 and 20 % RWC_4 h, respectively. Based on these data, a quantitative trait locus (QTL) analysis was performed. The impact of the identified QTLs on postharvest longevity of ten selected offspring was further evaluated. Three QTLs were identified: two major [positioned on linkage group 5 of the integrated consensus map (ICM 5) of both parents and on ICM 2 of the parent P867] and one putative minor (mapped to ICM 6 of both parents), explaining 32 % of the variability in the RWC_4 h. Low RWC_4 h was found to be a good proxy for eliminating the offspring with short vase life. This study constitutes a first step toward identifying the most likely regions for genes of interest controlling stomatal functioning in high RH-grown plants.

KW - marker-assisted selection

KW - water relations

KW - cut roses

KW - in-vitro

KW - traits

KW - environments

KW - conductance

KW - sensitivity

KW - improvement

KW - resistance

U2 - 10.1007/s11032-015-0365-7

DO - 10.1007/s11032-015-0365-7

M3 - Article

VL - 35

JO - Molecular Breeding

JF - Molecular Breeding

SN - 1380-3743

M1 - 172

ER -