QTL Analysis

C. Alonso-Blanco, M. Koornneef, J.W. van Ooijen

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

16 Citations (Scopus)

Abstract

The study of the intraspecific natural variation existing in Arabidopsis provides a useful resource for the dissection of its genome at the functional, ecological, and evolutionary levels. A major step in these studies is the identification and location of quantitative trait loci (QTLs) determining the variation for trait(s) of interest, so-called QTL analysis. Here we provide general methods to achieve this goal, including (1) the selection of wild accessions that can serve as parental lines for QTL mapping; (2) the development of mapping populations; (3) the construction of the required genomewide linkage maps of the populations; and (4) the mapping and characterization of QTLs affecting the trait(s).
Original languageEnglish
Title of host publicationMethods in molecular biology
Subtitle of host publicationArabidopsis Protocols
EditorsJ. Salinas, JJ Sanchez-Serrano
Place of PublicationInc. Totowa, New Jersey
PublisherHumana Press
Volume323
ISBN (Electronic)9781597450034
ISBN (Print)9781588293954
DOIs
Publication statusPublished - 2006

Publication series

NameMethods in Molecular Biology
PublisherHumana Press.
Volume323
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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