Purification and immuno-gold labeling of lily mottle virus from lily leaves

Yubao Zhang, Yajun Wang, Zhongkui Xie*, Ruoyu Wang, Zhihong Guo, Wopke van der Werf, Le Wang

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Lily mottle virus (LMoV) is prevalent in Lilium species worldwide causing dwarfing, flower breaking, and reduced bulb yield. In this paper, an easy to use and efficient procedure is described for purification of LMoV from lily leaves. The resulting sample is characterized by a 260/280 nm absorbance ratio of 1.20 at a concentration of 1.27 mg/ml. The procedure results in high protein purity and particle integrity as shown by UV-spectrophotometry, polyacrylamide gel electrophoresis (PAGE), Western blotting, reverse transcriptase (RT)-PCR and transmission electron microscopy (TEM) in combination with immuno-gold labeling. This is the first time that an immuno-gold labeling (IGL) assay was performed to identify a virus of lily. Purified products can be used as a source of antigen in the preparation of antibodies against LMoV and may assist in the development of a diagnostic test for LMoV and in epidemiological surveys.

Original languageEnglish
Pages (from-to)33-38
JournalJournal of Virological Methods
Volume232
DOIs
Publication statusPublished - 2016

Keywords

  • Immuno-gold labeling assay
  • Lily mottle virus
  • Purification
  • Transmission electron microscope

Fingerprint Dive into the research topics of 'Purification and immuno-gold labeling of lily mottle virus from lily leaves'. Together they form a unique fingerprint.

Cite this