TY - JOUR
T1 - Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3
AU - Reitsma, Marit
AU - Bastiaan-Net, Shanna
AU - Sforza, Stefano
AU - Van Der Valk, J.P.M.
AU - Van Gerth Van Wijk, Roy
AU - Savelkoul, H.F.J.
AU - De Jong, N.W.
AU - Wichers, H.J.
PY - 2016
Y1 - 2016
N2 - In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.
AB - In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.
KW - Ana o 1
KW - Ana o 2
KW - Ana o 3
KW - cashew
KW - tree nut allergy
U2 - 10.1021/acs.jafc.5b04401
DO - 10.1021/acs.jafc.5b04401
M3 - Article
AN - SCOPUS:84958093477
SN - 0021-8561
VL - 64
SP - 1191
EP - 1201
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 5
ER -