Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3

Marit Reitsma, Shanna Bastiaan-Net, Stefano Sforza, J.P.M. Van Der Valk, Roy Van Gerth Van Wijk, H.F.J. Savelkoul, N.W. De Jong, H.J. Wichers

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.

Original languageEnglish
Pages (from-to)1191-1201
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number5
DOIs
Publication statusPublished - 2016

Fingerprint

Anacardium
Anacardium occidentale
cashew nuts
Anas
Globulins
allergens
Allergens
Immunoglobulin E
Purification
Glycoproteins
Epitope Mapping
Electrophoresis
Sodium Dodecyl Sulfate
Epitopes
Polyacrylamide Gel Electrophoresis
Albumins
Proteins
Coloring Agents
Western Blotting
globulins

Keywords

  • Ana o 1
  • Ana o 2
  • Ana o 3
  • cashew
  • tree nut allergy

Cite this

Reitsma, Marit ; Bastiaan-Net, Shanna ; Sforza, Stefano ; Van Der Valk, J.P.M. ; Van Gerth Van Wijk, Roy ; Savelkoul, H.F.J. ; De Jong, N.W. ; Wichers, H.J. / Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3. In: Journal of Agricultural and Food Chemistry. 2016 ; Vol. 64, No. 5. pp. 1191-1201.
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abstract = "In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.",
keywords = "Ana o 1, Ana o 2, Ana o 3, cashew, tree nut allergy",
author = "Marit Reitsma and Shanna Bastiaan-Net and Stefano Sforza and {Van Der Valk}, J.P.M. and {Van Gerth Van Wijk}, Roy and H.F.J. Savelkoul and {De Jong}, N.W. and H.J. Wichers",
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Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3. / Reitsma, Marit; Bastiaan-Net, Shanna; Sforza, Stefano; Van Der Valk, J.P.M.; Van Gerth Van Wijk, Roy; Savelkoul, H.F.J.; De Jong, N.W.; Wichers, H.J.

In: Journal of Agricultural and Food Chemistry, Vol. 64, No. 5, 2016, p. 1191-1201.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3

AU - Reitsma, Marit

AU - Bastiaan-Net, Shanna

AU - Sforza, Stefano

AU - Van Der Valk, J.P.M.

AU - Van Gerth Van Wijk, Roy

AU - Savelkoul, H.F.J.

AU - De Jong, N.W.

AU - Wichers, H.J.

PY - 2016

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AB - In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.

KW - Ana o 1

KW - Ana o 2

KW - Ana o 3

KW - cashew

KW - tree nut allergy

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SP - 1191

EP - 1201

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

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