Purification and Characterization of Anacardium occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3

Marit Reitsma, Shanna Bastiaan-Net, Stefano Sforza, J.P.M. Van Der Valk, Roy Van Gerth Van Wijk, H.F.J. Savelkoul, N.W. De Jong, H.J. Wichers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

In this study a fast and simple purification procedure for the three known allergens from cashew (7S globulin Ana o 1, 11S globulin Ana o 2, and 2S albumin Ana o 3) is described. The purified allergens are characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot, glycoprotein stain, and protein identification. The purified proteins still bind IgE, and this IgE binding varied between different pools of patient serum. Ana o 1 was found to be a glycoprotein. Ana o 3 has been studied more in detail to identify both the small and large subunits, both displaying microheterogeneity, and epitope mapping of Ana o 3 has been performed.

Original languageEnglish
Pages (from-to)1191-1201
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number5
DOIs
Publication statusPublished - 2016

Keywords

  • Ana o 1
  • Ana o 2
  • Ana o 3
  • cashew
  • tree nut allergy

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