PUFAs acutely affect triacylglycerol-derived skeletal muscle fatty acid uptake and increase postprandial insulin sensitivity

A. Jans, E. Konings, G.H. Goossens, F.G. Bouwman, C.C. Moors, M.V. Boekschoten, L.A. Afman, M.R. Muller, E.C. Mariman, E.E. Blaak

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Background: Dietary fat quality may influence skeletal muscle lipid processing and fat accumulation, thereby modulating insulin sensitivity. Objective: The objective was to examine the acute effects of meals with various fatty acid (FA) compositions on skeletal muscle FA processing and postprandial insulin sensitivity in obese, insulin-resistant men. Design: In a single-blind, randomized, crossover study, 10 insulin-resistant men consumed 3 high-fat mixed meals (2.6 MJ), which were high in SFAs, MUFAs, or PUFAs. Fasting and postprandial skeletal muscle FA processing was examined by measuring differences in arteriovenous concentrations across the forearm muscle. [H-2(2)]Palmitate was infused intravenously to label endogenous triacylglycerol and FFAs in the circulation, and [U-C-13]palmitate was added to the meal to label chylomicron-triacylglycerol. Skeletal muscle biopsy samples were taken to assess intramuscular lipid metabolism and gene expression. Results: Insulin and glucose responses (AUC) after the SFA meal were significantly higher than those after the PUFA meal (P = 0.006 and 0.033, respectively). Uptake of triacylglycerol-derived FAs was lower in the postprandial phase after the PUFA meal than after the other meals (AUC(60-240); P = 0.02). The fractional synthetic rate of the triacylglycerol, diacylglycerol, and phospholipid pool was higher after the MUFA meal than after the SFA meal. PUFA induced less transcriptional downregulation of oxidative pathways than did the other meals. Conclusion: PUFAs reduced triacylglycerol-derived skeletal muscle FA uptake, which was accompanied by higher postprandial insulin sensitivity, a more transcriptional oxidative phenotype, and altered intra-myocellular lipid partitioning and may therefore be protective against the development of insulin resistance. This trial was registered at clinicaltrials.gov as NCT01466816. Am J Clin Nutr 2012;95:825-36.
LanguageEnglish
Pages825-836
JournalAmerican Journal of Clinical Nutrition
Volume95
DOIs
Publication statusPublished - 2012

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Meals
Insulin Resistance
Skeletal Muscle
Triglycerides
Fatty Acids
Palmitates
Insulin
Area Under Curve
Fats
Lipids
Chylomicrons
Dietary Fats
Diglycerides
Lipid Metabolism
Forearm
Cross-Over Studies
Fasting
Phospholipids
Down-Regulation
Phenotype

Keywords

  • beta-cell function
  • olive oil
  • metabolic syndrome
  • adipose-tissue
  • resistant subjects
  • glucose-tolerance
  • gene-expression
  • protein-content
  • soybean-oil
  • obesity

Cite this

Jans, A. ; Konings, E. ; Goossens, G.H. ; Bouwman, F.G. ; Moors, C.C. ; Boekschoten, M.V. ; Afman, L.A. ; Muller, M.R. ; Mariman, E.C. ; Blaak, E.E. / PUFAs acutely affect triacylglycerol-derived skeletal muscle fatty acid uptake and increase postprandial insulin sensitivity. In: American Journal of Clinical Nutrition. 2012 ; Vol. 95. pp. 825-836.
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abstract = "Background: Dietary fat quality may influence skeletal muscle lipid processing and fat accumulation, thereby modulating insulin sensitivity. Objective: The objective was to examine the acute effects of meals with various fatty acid (FA) compositions on skeletal muscle FA processing and postprandial insulin sensitivity in obese, insulin-resistant men. Design: In a single-blind, randomized, crossover study, 10 insulin-resistant men consumed 3 high-fat mixed meals (2.6 MJ), which were high in SFAs, MUFAs, or PUFAs. Fasting and postprandial skeletal muscle FA processing was examined by measuring differences in arteriovenous concentrations across the forearm muscle. [H-2(2)]Palmitate was infused intravenously to label endogenous triacylglycerol and FFAs in the circulation, and [U-C-13]palmitate was added to the meal to label chylomicron-triacylglycerol. Skeletal muscle biopsy samples were taken to assess intramuscular lipid metabolism and gene expression. Results: Insulin and glucose responses (AUC) after the SFA meal were significantly higher than those after the PUFA meal (P = 0.006 and 0.033, respectively). Uptake of triacylglycerol-derived FAs was lower in the postprandial phase after the PUFA meal than after the other meals (AUC(60-240); P = 0.02). The fractional synthetic rate of the triacylglycerol, diacylglycerol, and phospholipid pool was higher after the MUFA meal than after the SFA meal. PUFA induced less transcriptional downregulation of oxidative pathways than did the other meals. Conclusion: PUFAs reduced triacylglycerol-derived skeletal muscle FA uptake, which was accompanied by higher postprandial insulin sensitivity, a more transcriptional oxidative phenotype, and altered intra-myocellular lipid partitioning and may therefore be protective against the development of insulin resistance. This trial was registered at clinicaltrials.gov as NCT01466816. Am J Clin Nutr 2012;95:825-36.",
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PUFAs acutely affect triacylglycerol-derived skeletal muscle fatty acid uptake and increase postprandial insulin sensitivity. / Jans, A.; Konings, E.; Goossens, G.H.; Bouwman, F.G.; Moors, C.C.; Boekschoten, M.V.; Afman, L.A.; Muller, M.R.; Mariman, E.C.; Blaak, E.E.

In: American Journal of Clinical Nutrition, Vol. 95, 2012, p. 825-836.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - PUFAs acutely affect triacylglycerol-derived skeletal muscle fatty acid uptake and increase postprandial insulin sensitivity

AU - Jans, A.

AU - Konings, E.

AU - Goossens, G.H.

AU - Bouwman, F.G.

AU - Moors, C.C.

AU - Boekschoten, M.V.

AU - Afman, L.A.

AU - Muller, M.R.

AU - Mariman, E.C.

AU - Blaak, E.E.

PY - 2012

Y1 - 2012

N2 - Background: Dietary fat quality may influence skeletal muscle lipid processing and fat accumulation, thereby modulating insulin sensitivity. Objective: The objective was to examine the acute effects of meals with various fatty acid (FA) compositions on skeletal muscle FA processing and postprandial insulin sensitivity in obese, insulin-resistant men. Design: In a single-blind, randomized, crossover study, 10 insulin-resistant men consumed 3 high-fat mixed meals (2.6 MJ), which were high in SFAs, MUFAs, or PUFAs. Fasting and postprandial skeletal muscle FA processing was examined by measuring differences in arteriovenous concentrations across the forearm muscle. [H-2(2)]Palmitate was infused intravenously to label endogenous triacylglycerol and FFAs in the circulation, and [U-C-13]palmitate was added to the meal to label chylomicron-triacylglycerol. Skeletal muscle biopsy samples were taken to assess intramuscular lipid metabolism and gene expression. Results: Insulin and glucose responses (AUC) after the SFA meal were significantly higher than those after the PUFA meal (P = 0.006 and 0.033, respectively). Uptake of triacylglycerol-derived FAs was lower in the postprandial phase after the PUFA meal than after the other meals (AUC(60-240); P = 0.02). The fractional synthetic rate of the triacylglycerol, diacylglycerol, and phospholipid pool was higher after the MUFA meal than after the SFA meal. PUFA induced less transcriptional downregulation of oxidative pathways than did the other meals. Conclusion: PUFAs reduced triacylglycerol-derived skeletal muscle FA uptake, which was accompanied by higher postprandial insulin sensitivity, a more transcriptional oxidative phenotype, and altered intra-myocellular lipid partitioning and may therefore be protective against the development of insulin resistance. This trial was registered at clinicaltrials.gov as NCT01466816. Am J Clin Nutr 2012;95:825-36.

AB - Background: Dietary fat quality may influence skeletal muscle lipid processing and fat accumulation, thereby modulating insulin sensitivity. Objective: The objective was to examine the acute effects of meals with various fatty acid (FA) compositions on skeletal muscle FA processing and postprandial insulin sensitivity in obese, insulin-resistant men. Design: In a single-blind, randomized, crossover study, 10 insulin-resistant men consumed 3 high-fat mixed meals (2.6 MJ), which were high in SFAs, MUFAs, or PUFAs. Fasting and postprandial skeletal muscle FA processing was examined by measuring differences in arteriovenous concentrations across the forearm muscle. [H-2(2)]Palmitate was infused intravenously to label endogenous triacylglycerol and FFAs in the circulation, and [U-C-13]palmitate was added to the meal to label chylomicron-triacylglycerol. Skeletal muscle biopsy samples were taken to assess intramuscular lipid metabolism and gene expression. Results: Insulin and glucose responses (AUC) after the SFA meal were significantly higher than those after the PUFA meal (P = 0.006 and 0.033, respectively). Uptake of triacylglycerol-derived FAs was lower in the postprandial phase after the PUFA meal than after the other meals (AUC(60-240); P = 0.02). The fractional synthetic rate of the triacylglycerol, diacylglycerol, and phospholipid pool was higher after the MUFA meal than after the SFA meal. PUFA induced less transcriptional downregulation of oxidative pathways than did the other meals. Conclusion: PUFAs reduced triacylglycerol-derived skeletal muscle FA uptake, which was accompanied by higher postprandial insulin sensitivity, a more transcriptional oxidative phenotype, and altered intra-myocellular lipid partitioning and may therefore be protective against the development of insulin resistance. This trial was registered at clinicaltrials.gov as NCT01466816. Am J Clin Nutr 2012;95:825-36.

KW - beta-cell function

KW - olive oil

KW - metabolic syndrome

KW - adipose-tissue

KW - resistant subjects

KW - glucose-tolerance

KW - gene-expression

KW - protein-content

KW - soybean-oil

KW - obesity

U2 - 10.3945/ajcn.111.028787

DO - 10.3945/ajcn.111.028787

M3 - Article

VL - 95

SP - 825

EP - 836

JO - American Journal of Clinical Nutrition

T2 - American Journal of Clinical Nutrition

JF - American Journal of Clinical Nutrition

SN - 0002-9165

ER -