Providencia stuartii Isolates from Greece

Co-Carriage of Cephalosporin (blaSHV-5, blaVEB-1), Carbapenem (blaVIM-1), and Aminoglycoside (rmtB) Resistance Determinants by a Multidrug-Resistant Outbreak Clone

Olga Oikonomou, Apostolos Liakopoulos, Lynette M. Phee, Jonathan Betts, Dik Mevius, David W. Wareham*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Providencia stuartii has emerged as an important nosocomial pathogen. We describe an outbreak due to a multidrug-resistant strain over a 4-month period in a critical care unit in Athens. Molecular typing revealed each of the isolates to be clonally related with coresistance to cephalosporins, carbapenems, aminoglycosides, and quinolones. Each isolate contained a 220-kb multi-replicon (IncA/C and IncR) conjugative plasmid encoding TEM-1, SHV-5, VEB-1, and VIM-1 β-lactamases and the 16S rDNA methylase RmtB. Antimicrobial therapy was unsuccessful in 3 of 6 cases, and resistance was readily transmissible to susceptible strains of Escherichia coli by transformation and conjugation. This highlights the clinical importance of P. stuartii and its ability to disseminate critical resistance determinants to other bacterial pathogens.

Original languageEnglish
Pages (from-to)379-386
JournalMicrobial Drug Resistance-Mechanisms Epidemiology and Disease
Volume22
Issue number5
DOIs
Publication statusPublished - 2016

Fingerprint

Providencia
Molecular Typing
Replicon
Carbapenems
Greece
Quinolones
Aminoglycosides
Cephalosporins
Critical Care
Ribosomal DNA
Disease Outbreaks
Plasmids
Clone Cells
Escherichia coli
Therapeutics

Cite this

@article{68158ad094734022b26a9f00e37dfd81,
title = "Providencia stuartii Isolates from Greece: Co-Carriage of Cephalosporin (blaSHV-5, blaVEB-1), Carbapenem (blaVIM-1), and Aminoglycoside (rmtB) Resistance Determinants by a Multidrug-Resistant Outbreak Clone",
abstract = "Providencia stuartii has emerged as an important nosocomial pathogen. We describe an outbreak due to a multidrug-resistant strain over a 4-month period in a critical care unit in Athens. Molecular typing revealed each of the isolates to be clonally related with coresistance to cephalosporins, carbapenems, aminoglycosides, and quinolones. Each isolate contained a 220-kb multi-replicon (IncA/C and IncR) conjugative plasmid encoding TEM-1, SHV-5, VEB-1, and VIM-1 β-lactamases and the 16S rDNA methylase RmtB. Antimicrobial therapy was unsuccessful in 3 of 6 cases, and resistance was readily transmissible to susceptible strains of Escherichia coli by transformation and conjugation. This highlights the clinical importance of P. stuartii and its ability to disseminate critical resistance determinants to other bacterial pathogens.",
author = "Olga Oikonomou and Apostolos Liakopoulos and Phee, {Lynette M.} and Jonathan Betts and Dik Mevius and Wareham, {David W.}",
year = "2016",
doi = "10.1089/mdr.2015.0215",
language = "English",
volume = "22",
pages = "379--386",
journal = "Microbial Drug Resistance-Mechanisms Epidemiology and Disease",
issn = "1076-6294",
publisher = "Mary Ann Liebert",
number = "5",

}

Providencia stuartii Isolates from Greece : Co-Carriage of Cephalosporin (blaSHV-5, blaVEB-1), Carbapenem (blaVIM-1), and Aminoglycoside (rmtB) Resistance Determinants by a Multidrug-Resistant Outbreak Clone. / Oikonomou, Olga; Liakopoulos, Apostolos; Phee, Lynette M.; Betts, Jonathan; Mevius, Dik; Wareham, David W.

In: Microbial Drug Resistance-Mechanisms Epidemiology and Disease, Vol. 22, No. 5, 2016, p. 379-386.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Providencia stuartii Isolates from Greece

T2 - Co-Carriage of Cephalosporin (blaSHV-5, blaVEB-1), Carbapenem (blaVIM-1), and Aminoglycoside (rmtB) Resistance Determinants by a Multidrug-Resistant Outbreak Clone

AU - Oikonomou, Olga

AU - Liakopoulos, Apostolos

AU - Phee, Lynette M.

AU - Betts, Jonathan

AU - Mevius, Dik

AU - Wareham, David W.

PY - 2016

Y1 - 2016

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AB - Providencia stuartii has emerged as an important nosocomial pathogen. We describe an outbreak due to a multidrug-resistant strain over a 4-month period in a critical care unit in Athens. Molecular typing revealed each of the isolates to be clonally related with coresistance to cephalosporins, carbapenems, aminoglycosides, and quinolones. Each isolate contained a 220-kb multi-replicon (IncA/C and IncR) conjugative plasmid encoding TEM-1, SHV-5, VEB-1, and VIM-1 β-lactamases and the 16S rDNA methylase RmtB. Antimicrobial therapy was unsuccessful in 3 of 6 cases, and resistance was readily transmissible to susceptible strains of Escherichia coli by transformation and conjugation. This highlights the clinical importance of P. stuartii and its ability to disseminate critical resistance determinants to other bacterial pathogens.

U2 - 10.1089/mdr.2015.0215

DO - 10.1089/mdr.2015.0215

M3 - Article

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SN - 1076-6294

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