Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig

Yasmin Eckstein; Barbara Kessler; Arne Hinrichs; Istvan Novak; Anne von Thaden; Timo Lorenzen; Birgit Rathkolb; Armin Scholz; Andreas Blutke; Sietse Jan Koopmans; Martin Hrabĕ de Angelis; Berit Østergaard Christoffersen; Eckhard Wolf; Simone Renner

doi:10.1016/j.xpro.2025.103774

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig

Yasmin Eckstein
, Barbara Kessler
, Arne Hinrichs
, Istvan Novak
, Anne von Thaden
, Timo Lorenzen
, Birgit Rathkolb
, Armin Scholz
, Andreas Blutke
, Sietse Jan Koopmans
, Martin Hrabĕ de Angelis
, Berit Østergaard Christoffersen
, Eckhard Wolf
, Simone Renner^*

^*Corresponding author for this work

Animal Nutrition

Research output: Contribution to journal › Article › Academic › peer-review

1 Citation (Scopus)

Abstract

The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.¹ and Renner et al.²

Original language	English
Article number	103774
Journal	STAR Protocols
Volume	6
Issue number	2
DOIs	https://doi.org/10.1016/j.xpro.2025.103774
Publication status	Published - 20 Jun 2025

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biotechnology and bioengineering
Metabolism
Model Organisms

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https://edepot.wur.nl/692699Licence: CC BY

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Eckstein, Y., Kessler, B., Hinrichs, A., Novak, I., von Thaden, A., Lorenzen, T., Rathkolb, B., Scholz, A., Blutke, A., Koopmans, S. J., de Angelis, M. H., Christoffersen, B. Ø., Wolf, E., & Renner, S. (2025). Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. STAR Protocols, 6(2), Article 103774. https://doi.org/10.1016/j.xpro.2025.103774

@article{2c57a88b4e56429887e907b36ced20fa,

title = "Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig",

abstract = "The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2",

keywords = "Biotechnology and bioengineering, Metabolism, Model Organisms",

author = "Yasmin Eckstein and Barbara Kessler and Arne Hinrichs and Istvan Novak and \{von Thaden\}, Anne and Timo Lorenzen and Birgit Rathkolb and Armin Scholz and Andreas Blutke and Koopmans, \{Sietse Jan\} and \{de Angelis\}, \{Martin Hrabĕ\} and Christoffersen, \{Berit {\O}stergaard\} and Eckhard Wolf and Simone Renner",

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day = "20",

doi = "10.1016/j.xpro.2025.103774",

language = "English",

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Eckstein, Y, Kessler, B, Hinrichs, A, Novak, I, von Thaden, A, Lorenzen, T, Rathkolb, B, Scholz, A, Blutke, A, Koopmans, SJ, de Angelis, MH, Christoffersen, BØ, Wolf, E & Renner, S 2025, 'Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig', STAR Protocols, vol. 6, no. 2, 103774. https://doi.org/10.1016/j.xpro.2025.103774

TY - JOUR

T1 - Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig

AU - Eckstein, Yasmin

AU - Kessler, Barbara

AU - Hinrichs, Arne

AU - Novak, Istvan

AU - von Thaden, Anne

AU - Lorenzen, Timo

AU - Rathkolb, Birgit

AU - Scholz, Armin

AU - Blutke, Andreas

AU - Koopmans, Sietse Jan

AU - de Angelis, Martin Hrabĕ

AU - Christoffersen, Berit Østergaard

AU - Wolf, Eckhard

AU - Renner, Simone

PY - 2025/6/20

Y1 - 2025/6/20

N2 - The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2

AB - The pig is a valuable animal model in diabetes research; however, standardized protocols are essential for evaluating in vivo metabolism. Here, we present a protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig. We describe steps for catheter implantation, testing of intravenous glucose tolerance, performance of hyperinsulinemic-euglycemic clamps (HECs) and hyperglycemic clamps (HGCs), and blood processing. We then detail procedures for analysis of plasma glucose, insulin, glucagon, and C-peptide concentrations as well as data analysis. For complete details on the use and execution of this protocol, please refer to Renner et al.1 and Renner et al.2

KW - Biotechnology and bioengineering

KW - Metabolism

KW - Model Organisms

U2 - 10.1016/j.xpro.2025.103774

DO - 10.1016/j.xpro.2025.103774

M3 - Article

AN - SCOPUS:105002662027

SN - 2666-1667

VL - 6

JO - STAR Protocols

JF - STAR Protocols

IS - 2

M1 - 103774

ER -

Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig

Abstract

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Keywords

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