Abstract
Arabidopsis thaliana cytosolic ribosomes are large complexes containing eighty-one distinct ribosomal proteins (r-proteins), four ribosomal RNAs (rRNA) and a plethora of associated (non-ribosomal) proteins. In plants, r-proteins of cytosolic ribosomes are each encoded by two to seven different expressed and similar genes, forming an r-protein family. Distinctions in the r-protein coding sequences of gene family members are a source of variation between ribosomes. We performed proteomic investigation of actively translating cytosolic ribosomes purified using both immunopurification and a classical sucrose cushion centrifugation-based protocol from plants of different developmental stages. Both 1D and 2D LC-MSE with data-independent acquisition as well as conventional data-dependent MS/MS procedures were applied. This approach provided detailed identification of 165 r-protein paralogs with high coverage based on proteotypic peptides. The detected r-proteins were the products of the majority (68%) of the 242 cytosolic r-proteins genes encoded by the genome. A total of 70 distinct r-proteins were identified. Based on these results and information from DNA microarray and ribosome footprint profiling studies a re-annotation of Arabidopsis r-proteins and genes is proposed. This compendium of the cytosolic r-protein proteome will serve as a template for future investigations on the dynamic structure and function of plant ribosomes.
Original language | English |
---|---|
Pages (from-to) | 436-449 |
Number of pages | 37 |
Journal | Journal of Proteomics |
Volume | 128 |
DOIs | |
Publication status | Published - 2015 |
Keywords
- A. thaliana
- Data-independent acquisition
- DIA
- LC-MS
- Paralogs
- Ribosomal protein
- Ribosomes