Proteome-wide characterization of seed aging in Arabidopsis. A comparison between artificial and natural aging protocols

L. Rajjou, Y. Lovigny, S.P.C. Groot, M. Belghazi, C. Job, D. Job

    Research output: Contribution to journalArticleAcademicpeer-review

    233 Citations (Scopus)

    Abstract

    A variety of mechanisms has been proposed to account for the extension of life span in seeds (seed longevity). In the present work, we have used Arabidopsis thaliana seeds as a model and carried out differential proteomics to investigate this trait, which is of both ecological and agricultural importance. In our system based on a controlled deterioration treatment (CDT), we compared seed samples treated for different periods of time, up to seven days. Germination tests showed a progressive decrease of germination vigor depending on the duration of CDT. Proteomic analyses revealed that this loss in seed vigor can be accounted for by protein changes in the dry seeds and by an inability of the low-vigor seeds to display a normal proteome during germination. Furthermore, the CDT strongly increased the extent of protein oxidation (carbonylation), which might induce a loss of functional properties of seed proteins and enzymes and/or enhance their susceptibility toward proteolysis. These results unveiled essential mechanisms for seed vigor as translational capacity, mobilization of seed storage reserves and detoxification efficiency. Finally, the present work shows that similar molecular events accompany artificial and natural seed aging.
    Original languageEnglish
    Pages (from-to)620-641
    JournalPlant Physiology
    Volume148
    DOIs
    Publication statusPublished - 2008

    Keywords

    • embryogenesis abundant protein
    • gene-expression programs
    • stored messenger-rna
    • oxidative stress
    • abscisic-acid
    • controlled deterioration
    • methionine biosynthesis
    • biotinylated protein
    • developmental-stages
    • disulfide-isomerase

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